1. 多肽产品
  2. 多肽及其衍生物
  3. 抑制剂与底物肽
  4. 酶底物
  5. Ac-WEHD-AFC

Ac-WEHD-AFC 是一种荧光 caspase-1 底物。Ac-WEHD-AFC 可检测 caspase-1 荧光活性,用于肿瘤和炎症的研究。

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Custom Peptide Synthesis

Ac-WEHD-AFC Chemical Structure

Ac-WEHD-AFC Chemical Structure

CAS No. : 210344-99-3

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Ac-WEHD-AFC 的其他形式现货产品:

Customer Review

Other Forms of Ac-WEHD-AFC:

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Ac-WEHD-AFC is a fluorogenic caspase-1 substrate. Ac-WEHD-AFC can measure caspase-1 fluorogenic activity and can be used for the research of tumor and inflammation[1].

体外研究
(In Vitro)

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Caspase-1 Fluorogenic Activity Assay[1]:
1. Incubate the cells according to your normal protocol.
2. Cells are transiently transfected with caspase-1 in combination with pcDNA3.1, Myc-cIAP2, Myc-cIAP1, or Myc-XIAP and collected 24 hr later.
3. Cells were lysed in CHEGG buffer before sonicating for 15 s at 60% amplitude (for cleavage assays).
4. Lysates were incubated with 10 mM of the fluorogenic caspase-1 substrate Ac-WEHD-AFC.
5. The release of free AFC was monitored continuously for 1 hr (excitation 380 nm, emission 460 nm) in 1 min intervals and expressed as arbitrary fluorescence units per minute.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

838.74

Formula

C38H37F3N8O11

CAS 号
Sequence Shortening

Ac-WEHD-{AFC}

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料
参考文献
  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
Ac-WEHD-AFC
目录号:
HY-P2617
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