DDX5 抗体 (YA2666)

目录号: HY-P82921: Data Sheet SDS COA 抗体使用指南
FAQs
技术支持

DDX5 Antibody (YA2666) 是一个兔来源、无偶联标记、抗 DDX5 的 IgG 单克隆抗体。

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规格	价格	是否有货
10 μL	￥470	In-stock
50 μL	￥1220	In-stock
100 μL	￥2000	In-stock
250 μL		询价

or 大包装询价

* Please select Quantity before adding items.

WB: 蛋白质免疫印迹;
IHC-P: 石蜡切片样本的免疫组织化学;
IHC-F: 冰冻切片样本的免疫组织化学;
ICC/IF: 细胞免疫荧光;
IF-Tissue: 组织免疫荧光;
mIHC: 多重荧光免疫组化;
IP: 免疫沉淀;
ChIP: 染色质免疫沉淀;
FC: 流式细胞术;
ELISA: 酶联免疫吸附试验

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背景信息
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描述

DDX5 Antibody (YA2666) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DDX5.

宿主

Rabbit

克隆性

Recombinant, Monoclonal

分子量

Predicted band size: 69 kDa;

Observed band size: 69 kDa

请注意：因蛋白存在修饰或聚体等情况，以实测为准，预测仅为参考。

反应种属

Human, Mouse, Rat

蛋白数据库

P17844

基因 ID

1655 [NCBI]

免疫原

A synthetic peptide of human DDX5 aa9-21.

应用 & 推荐
稀释比例

应用	稀释比
WB WB: 蛋白质免疫印迹	1:500-1:1000
IHC-P IHC-P: 石蜡切片样本的免疫组织化学	1:50-1:100
IHC-F IHC-F: 冰冻切片样本的免疫组织化学	1:50-1:100
ICC/IF ICC/IF: 细胞免疫荧光	1:50-1:200
IP IP: 免疫沉淀	1:20

敏感性	Endogenous	纯度	Affinity Purified
偶联	Non-conjugated	修饰	Unmodified
同型	IgG

性状

液体

组分

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片

ALL ICC IHC-P

Immunocytochemistry analysis of NIH/3T3 cells labeling DDX5 Antibody (HY-P82921) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with DDX5 Antibody (HY-P82921) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of SH-SY5Y cells labeling DDX5 Antibody (HY-P82921) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with DDX5 Antibody (HY-P82921) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using DDX5 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using DDX5 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

背景

功能：参与前体 mRNA 剪接的替代调控；其 RNA 解旋酶活性对于增加 tau 蛋白第 10 外显子的插入是必需的，且该过程依赖于 RBM4。结合于 tau 蛋白前体 mRNA 第 10 外显子下游的茎环区域。单链 RNA 可显著刺激 ATP 水解速率。参与转录调控；其功能独立于 RNA 解旋酶活性。是雄激素受体 AR 的转录共激活因子，但可能不是 ESR1 的转录共激活因子。与 DDX17 和 SRA1 RNA 协同激活 MYOD1 转录活性，并参与骨骼肌分化。是 p53/TP53 的转录共激活因子，参与 p53/TP53 对 DNA 损伤的转录反应以及 p53/TP53 依赖性细胞凋亡。是 RUNX2 的转录共激活因子，参与成骨细胞分化的调控。它以启动子特异性的方式发挥转录抑制作用；其功能可能涉及与组蛋白去乙酰化酶 (如 HDAC1) 的结合。作为大型 PER 复合物的组成部分，它参与抑制昼夜节律靶基因 (如 PER1 和 NR1D1) 的 3'转录终止，并调控昼夜节律。

亚细胞定位：细胞核；细胞核，核仁；核斑；细胞质

异构体 & 翻译后修饰：P17844 有两种异构体：P17844-1：69148 Da (预测值)；P17844-2：60563 Da (预测值)。
Arg-502 被二甲基化，可能生成不对称二甲基精氨酸；SUMO 化；SUMO 化由 PIAS1 促进，可促进与 HDAC1 的相互作用和转录抑制活性。SUMO 化还显著提高稳定性，并降低多聚泛素化；多聚泛素化会导致蛋白酶体降解；在细胞周期的 G1/S 期磷酸化较弱，在 G2/M 期磷酸化程度更高，尤其是在 Thr 和 Tyr 残基处。

亚基：在剪接体 C 复合物中发现 (PubMed:11991638)。核糖核蛋白复合物的组成部分，该复合物包含 mRNA 和 RNA 结合蛋白，包括 DDX5、HNRNPH2 和 SRSF1 以及剪接调节因子 ARVCF (PubMed:24644279)。

RRID

AB_3105013

反应种属数据库

Entrez Gene: 1655 Human ; 13207 Mouse ; 287765 Rat

SwissProt: P17844 Human ; Q61656 Mouse ;

OMIM: 180630 Human

研究领域

Epigenetics and Nuclear Signaling

中文名

DDX5 抗体 (YA2666)

同用名

DDX5; G17P1; HELR; HLR1; Probable ATP-dependent RNA helicase DDX5; DEAD box protein 5; RNA helicase p68

文件资料

Select Batch:

Data Sheet (443 KB) SDS (251 KB)

COA (193 KB)

抗体使用指南 (1083 KB)

DDX5 Antibody (YA2666) 相关分类

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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