1. 抗体
  2. 一抗
  3. 单克隆抗体 流式抗体
  4. E-Cadherin 抗体 (YA778)

E-Cadherin 抗体 (YA778)

目录号: HY-P80112
COA 抗体使用指南 技术支持

E-Cadherin Antibody (YA778) 是一个小鼠来源、无偶联标记、抗 E-Cadherin 的 IgG1 单克隆抗体。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
10 μL ¥470 In-stock
50 μL ¥1220 In-stock
100 μL ¥2000 In-stock
250 μL   询价  

* Please select Quantity before adding items.

MCE 顾客使用本产品发表的科研文献

  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验
  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

E-Cadherin Antibody (YA778) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to E-Cadherin.

宿主

Mouse

克隆性

Monoclonal

分子量
Predicted band size: 97 kDa;
Observed band size: 130 kDa
请注意:因蛋白存在修饰或聚体等情况,以实测为准,预测仅为参考。
反应种属
Human, Mouse
蛋白数据库
基因 ID
免疫原

Synthetic peptide corresponding to Mouse E-Cadherin.AA range:350-550.

应用 & 推荐
稀释比例
应用 稀释比
WB
WB: 蛋白质免疫印迹
1:500-1:1000
IHC-P
IHC-P: 石蜡切片样本的免疫组织化学
1:200
敏感性 Endogenous 纯度 Protein A affinity purified.
偶联 Non-conjugated 修饰 Unmodified
同型 IgG1  
性状

液体

组分

Supplied in 1*PBS (pH7.4), 0.2% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片
ALL WB IHC-P
  • Western blot analysis of extracts from A431(lane 2(20μg) and A431(lane 3(40μg) using E-Cadherin (HY-P80112) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using E-Cadherin Antibody (YA778). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80112, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using E-Cadherin Antibody (YA778). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80112, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

背景
功能:钙黏蛋白是钙依赖性细胞黏附蛋白 (PubMed:11976333)。它们优先以同型方式相互连接细胞;因此,钙黏蛋白可能有助于异质细胞类型的分选。CDH1 参与调节上皮细胞的细胞间黏附、迁移和增殖的机制 (PubMed:11976333)。它通过与 AMOTL2 相互作用,促进放射状肌动蛋白纤维结构的组织和细胞对收缩力的反应,AMOTL2 促进放射状肌动蛋白纤维锚定到细胞膜上的 CDH1 连接复合物 (基于相似性)。它通过促进 DSG2 和 DSP 募集到桥粒斑块,在桥粒细胞间连接形成的早期阶段发挥作用 (PubMed:29999492)。它具有强大的侵袭抑制作用。它是整合素α-E/β-7 的配体; E-钙黏蛋白/CTF2 促进 Aβ前体非淀粉样蛋白生成降解。对 APP C99 和 C83 的产生具有强烈的抑制作用;(微生物感染) 作为单核细胞增生李斯特菌的受体;内吞素 A (InlA) 与该蛋白结合并促进细菌的摄取。
亚细胞定位:细胞连接,黏着连接;细胞膜;I 型单次跨膜蛋白;内体;高尔基体,反式高尔基网络;细胞质;细胞连接,桥粒
表达水平:
组织特异性:在肉芽肿巨噬细胞中表达 (蛋白水平) (PubMed:27760340) 。在皮肤中表达 (蛋白水平) (PubMed:22294297) 。在肝脏中表达 (PubMed:3263290) 。

诱导:在角质形成细胞中,周期性机械应变后数小时内即可诱导该基因表达 (PubMed:31835537) 。该基因的表达受MACROD1抑制 (PubMed:17893710) 。
亚基:同源二聚体;二硫键连接 (PubMed:11856755)。E-钙黏蛋白/连环蛋白黏附复合物的组成部分,该复合物至少由 E-钙黏蛋白/CDH1、β-连环蛋白/CTNNB1 或 γ-连环蛋白/JUP 组成,并且可能包含 α-连环蛋白/CTNNA1。
RRID
反应种属数据库
研究领域

Signal Transduction

中文名
E-Cadherin 抗体 (YA778)
文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
E-Cadherin Antibody (YA778)
目录号:
HY-P80112
需求量: