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  2. 一抗
  3. 单克隆抗体 重组抗体 流式抗体
  4. GLB1/Beta-galactosidase 抗体 (YA2816)

GLB1/Beta-galactosidase 抗体 (YA2816)

目录号: HY-P83071
COA 抗体使用指南 技术支持

GLB1/Beta-galactosidase Antibody (YA2816) 是一个兔来源、无偶联标记、抗 GLB1/Beta-galactosidase 的 IgG 单克隆抗体。

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规格 价格 是否有货 数量
10 μL ¥470 In-stock
50 μL ¥1220 In-stock
100 μL ¥2000 In-stock
250 μL   询价  

* Please select Quantity before adding items.

  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验
  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

GLB1/Beta-galactosidase Antibody (YA2816) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GLB1/Beta-galactosidase.

宿主

Rabbit

克隆性

Recombinant, Monoclonal

分子量
Predicted band size: 76 kDa;
Observed band size: 95 kDa
请注意:因蛋白存在修饰或聚体等情况,以实测为准,预测仅为参考。
反应种属
Human, Mouse, Rat
蛋白数据库
基因 ID
免疫原

A synthesized peptide derived from human GLB1 aa621-677/677.

应用 & 推荐
稀释比例
应用 稀释比
WB
WB: 蛋白质免疫印迹
1:500-1:1000
FC
FC: 流式细胞术
1:50-1:100
敏感性 Endogenous 纯度 Affinity Purified
偶联 Non-conjugated 修饰 Unmodified
同型 IgG  
性状

液体

组分

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片
ALL WB FC
  • Western blot analysis of extracts from C6(lane 2(20ug) , Hela(lane 3(20ug) and K562(lane 4(20ug) using GLB1 Antibody (HY-P83071) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Flow cytometric analysis of 1X10^6 A549 cells labeling GLB1 Antibody (YA2816) (HY-P82032, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

背景
功能:可从神经节苷脂、糖蛋白和糖胺聚糖中裂解β-连接的末端半乳糖残基;不具有β-半乳糖苷酶催化活性,但在细胞外弹性纤维的形成 (弹性蛋白生成) 和结缔组织的发育中发挥功能性作用。似乎与弹性蛋白结合蛋白 (EBP) 相同,EBP 是表达于成纤维细胞、平滑肌细胞、软骨母细胞、白细胞和某些癌细胞类型的非整合素细胞表面受体的主要成分。在产生弹性蛋白的细胞中,它与细胞内的原弹性蛋白结合,并作为一种循环分子伴侣发挥作用,促进原弹性蛋白的分泌及其组装成弹性纤维。
亚细胞定位:溶酶体;细胞质,核周区
表达水平:
组织特异性:在胎盘中检测到 (蛋白质水平) (PubMed:8383699) 。在成纤维细胞和睾丸中检测到 (PubMed:2511208) 。
异构体 & 翻译后修饰:P16278 有 3 个异构体:P16278-1:76075 Da (预测);P16278-2:60536 Da (预测);P16278-3:72751 Da (预测)。
亚基:同源二聚体 (PubMed:22128166)。可能形成更高级的多聚体 (可能)。
RRID
反应种属数据库
研究领域

Tags & Cell Markers

中文名
GLB1/Beta-galactosidase 抗体 (YA2816)
同用名
GLB1; ELNR1; Beta-galactosidase; Acid beta-galactosidase; Lactase; Elastin receptor 1
文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Inquiry Information

产品名称:
GLB1/Beta-galactosidase Antibody (YA2816)
目录号:
HY-P83071
需求量: