HDAC3 抗体 (YA392)

HDAC3 Antibody (YA392) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to HDAC3.

HDAC3 蛋白是一种具有多种底物特异性的组蛋白脱乙酰酶，可催化位于核心组蛋白（H2A、H2B、H3 和 H4）N 末端区域的赖氨酸残基以及各种非组蛋白底物的脱乙酰化。这种酶活性赋予表观遗传抑制标签，并在转录调节、细胞周期进程和发育事件中发挥关键作用。HDAC3 在大型多蛋白复合物中发挥作用，通过使增强子元件上的 H3“Lys-27”(H3K27) 去乙酰化、抵消 EP300 乙酰转移酶活性并抑制近端基因表达来参与 BCL6 转录抑制活性。此外，HDAC3 充当分子伴侣，促进磷酸化 NR2C1 穿梭至 PML 体进行 sumoylation（通过相似性）。它有助于 NFE2L2 介导的 HMOX1 转录因子基因表达，促进内皮细胞在血流紊乱和氧化应激下存活。在生物钟中，HDAC3 调节激活和抑制阶段，影响 BMAL1 的泛素化，阻断 FBXL3 介导的 CRY1/2 泛素化，并促进 CRY1 和 BMAL1 的相互作用（通过相似性）。作为一种多功能脱乙酰酶，HDAC3 靶向非组蛋白，如 KAT5、MEF2D、MAPK14 和 RARA，充当 RARA 的辅阻遏物，并介导其脱乙酰化以抑制 RARE DNA 元件结合。值得注意的是，HDAC3 还表现出蛋白质赖氨酸脱酰酶活性，从赖氨酸残基中去除酰基，例如 (2E)-丁烯酰基（巴豆酰基）和 2-羟基异丁酰基（2-羟基异丁酰基），分别有助于蛋白质去巴豆酰化和去-2-羟基异丁酰化。这种多样化的活动凸显了 HDAC3 在表观遗传调控和细胞过程中的核心作用。

Free

O15379

Predicted band size: 49 kDa; Observed band size: 49 kDa

Protein A affinity purified.

Cytoplasm, Nucleus.

Non-conjugated

Unmodified

AB_3102319

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:1,000-1:5,000 ;ICC/IF: 1:50 ;IHC-P: 1:50-1:1,000 ;IP: Use at an assay dependent concentration.

• 
  Western blot analysis of extracts from Hela(lane 2(20μg) , HEK293 (lane 3(20μg) and NIH3T3 (lane 4(20μg) using HDAC3(HY-P80152) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of NIH/3T3 cells labeling HDAC3 with HDAC3 Antibody (HY-P80152) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with HDAC3 Antibody (HY-P80152) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of Hela cells labeling HDAC3 with HDAC3 Antibody (HY-P80152)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with HDAC3 Antibody (HY-P80152) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunohistochemical analysis of paraffin-embedded Rat kidney tissue using HDAC3 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80152, 1/400) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Rat kidney tissue using HDAC3 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80152, 1/400) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, ICC/IF, IHC-P, IP

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide within C-terminal human HDAC3.