1. 抗体
  2. 一抗
  3. 单克隆抗体 重组抗体
  4. IKB alpha 抗体 (YA350)

IKB alpha 抗体 (YA350)

目录号: HY-P80718
COA 抗体使用指南 技术支持

IKB alpha Antibody (YA350) 是一个兔来源、无偶联标记、抗 IKB alpha 的 IgG 单克隆抗体。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
10 μL ¥470 In-stock
50 μL ¥1220 In-stock
100 μL ¥2000 In-stock
250 μL   询价  

* Please select Quantity before adding items.

MCE 顾客使用本产品发表的科研文献

  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验
  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

IKB alpha Antibody (YA350) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IKB alpha.

宿主

Rabbit

克隆性

Recombinant, Monoclonal

分子量
Predicted band size: 36 kDa;
Observed band size: 39 kDa
请注意:因蛋白存在修饰或聚体等情况,以实测为准,预测仅为参考。
反应种属
Human, Mouse, Rat
蛋白数据库
基因 ID
免疫原

Synthetic peptide corresponding to Human IKB alpha.The exact sequence is proprietary to MCE.

应用 & 推荐
稀释比例
应用 稀释比
WB
WB: 蛋白质免疫印迹
1:500-1:1000
IHC-P
IHC-P: 石蜡切片样本的免疫组织化学
1:50-1:100
IP
IP: 免疫沉淀
1:20
敏感性 Endogenous 纯度 affinity purified
偶联 Non-conjugated 修饰 Unmodified
同型 IgG  
性状

液体

组分

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片
ALL WB IHC-P
  • Western blot analysis of extracts from Hela(lane 2(20μg) , C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) using IKB alpha Antibody (HY-P80718) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using IKB alpha Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using IKB alpha Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

背景
功能:通过掩盖核定位信号,将 REL (RELA/p65 和 NFKB1/p50) 二聚体捕获在细胞质中,从而抑制二聚体 NF-κB/REL 复合物的活性 (PubMed:1493333, PubMed:36651806, PubMed:7479976)。在免疫和促炎反应刺激细胞时,该蛋白发生磷酸化,促进泛素化和降解,使二聚体 RELA 易位至细胞核并激活转录 (PubMed:7479976, PubMed:7628694, PubMed:7796813, PubMed:7878466)。
亚细胞定位:细胞质;细胞核
表达水平:
诱导:在贴壁单核细胞中诱导
亚基:与 RELA 相互作用;该相互作用需要核输入信号 (PubMed:1493333)。至少包含 CHUK、IKBKB、NFKBIA、RELA、ELP1 和 MAP3K14 的 70-90 kDa 复合物的组成部分 (PubMed:9751059)。与 NKIRAS1 和 NKIRAS2 相互作用 (PubMed:10657303)。与 RWDD3 的异构体 1 和异构体 2 相互作用;该相互作用增强 SUMO 化 (PubMed:17956732, PubMed:23469069)。与 PRMT2 相互作用 (PubMed:16648481)。与血小板中的 PRKACA 相互作用;该相互作用可被凝血酶和胶原蛋白破坏 (PubMed:20356841)。与 MEFV 相互作用 (PubMed:18577712)。与 DDRGK1 相互作用;正向调控 NFKBIA 的磷酸化和降解 (PubMed:23675531)。与 HNRNPA2B1 相互作用;该相互作用可能由 HNRNPA2B1 的 RRM2 结构域介导,并且 HNRNPA2B1 可能同时与 FAM76B 和 NFKBIA 或 NFKBIE 相互作用形成复合物 (PubMed:37643469)。
RRID
反应种属数据库
研究领域

Epigenetics and Nuclear Signaling

中文名
IKB alpha 抗体
同用名
NFKBIA; IKBA; MAD3; NFKBI; NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3
文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
IKB alpha Antibody (YA350)
目录号:
HY-P80718
需求量: