IL-1 beta 抗体 (YA345)

IL-1 beta Antibody (YA345) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IL-1 beta.

IL-1 β 蛋白是一种有效的促炎细胞因子，因其在协调免疫反应中的多种作用而受到认可。IL-1β最初被认为是一种主要的内源性热原，可诱导一系列炎症事件，包括前列腺素合成、中性粒细胞流入和激活、T细胞和B细胞激活、细胞因子产生以及成纤维细胞增殖和胶原蛋白产生。它在免疫细胞分化中发挥着关键作用，促进 T 细胞的 Th17 分化，并与 IL-12 协同诱导辅助性 T 1 (Th1) 细胞合成 IFNG。此外，IL-1β 通过诱导 VEGF 产生，与 TNF 和 IL-6 协同作用，促进血管生成。值得注意的是，它在细胞焦亡下游炎症转导中发挥着关键作用，通过gasdermin-D (GSDMD) 孔特异性释放到细胞外环境中。在微生物感染的情况下，IL-1β充当皮肤中化脓性链球菌感染的传感器，被化脓性链球菌SpeB蛋白酶裂解和激活，导致炎症反应，从而在侵袭性皮肤感染期间抑制细菌生长。然而，SpeB 对 IL-1β 的裂解具有双重作用，通过破坏微生物群介导的定植抵抗来促进鼻咽链球菌感染。

Free

P01584

Predicted band size: 31 kDa; Observed band size: 17 kDa

Protein A affinity purified.

Cytoplasm, Lysosome, extracellular exosome, Secreted.

Non-conjugated

Unmodified

AB_3102576

Immunology

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:1000; ICC/IF: 1:50-1:200; IHC-P: 1:50-1:200; FC: 1:50-1:100

• 
  Western blot analysis of extracts from THP-1 (lane 2(20μg) and THP-1 (lane 3(20μg) , LPS treated (HY-D1056, 100ng/mL, 2hrs) as indicated, using IL-1 beta(HY-P80503) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue using IL-1 beta Antibody (YA345). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80503, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue using IL-1 beta Antibody (YA345). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80503, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, ICC/IF, IHC-P, FC

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide within C-terminal human IL1 beta.