PKC gamma 抗体 (YA125)

PKC gamma Antibody (YA125) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PKC gamma.

PKC gamma 是一种钙离子激活、依赖磷脂和二酰基甘油 (DAG) 的丝氨酸/苏氨酸蛋白激酶，在神经元细胞和眼组织中发挥多种功能。它通过增加质膜相关 GRIA4 的表达来调节神经元受体 GRIA4/GLUR4 和 GRIN1/NMDAR1 的功能，影响对阿片类药物、疼痛和酒精的敏感性，介导缺血后的突触功能和细胞存活，并在氧化应激后抑制间隙连接活性。在初级小脑神经元中，PKC gamma 位于代谢型谷氨酸受体 GRM5/MGLUR5 下游，磷酸化 GRIN1/NMDAR1 受体，该受体在突触可塑性、突触发生、兴奋性毒性和学习记忆中起关键作用。PKC gamma 可能参与海马长时程增强 (LTP) 的调节，但对突触可塑性过程可能并非必需。在脊髓中，它可能参与 μ 型阿片受体介导的 G 蛋白激活的脱敏，并对吗啡诱导的强化效应的发育和/或维持至关重要。此外，PKC gamma 通过参与导致阿片受体磷酸化和降解的信号通路来调节 μ 型阿片受体的功能，并可能促进慢性吗啡诱导的伤害性处理变化。它在神经病理性疼痛机制中发挥作用，并参与外周炎症引起的异常性疼痛的维持。PKC gamma 通过介导乙醇的行为效应及其对 GABA(A) 受体的影响，在乙醇的初始敏感性和耐受性中起重要作用。在脑缺血期间和之后，它调节突触膜中的神经传递和细胞存活，并参与胰岛素诱导的坏死抑制，这是最小化缺血性损伤的重要机制。PKC gamma 在小脑发育过程中对浦肯野细胞的多重攀爬纤维消除是必需的。在过氧化氢处理的晶状体上皮细胞中，PKC gamma 被激活并磷酸化连接蛋白 -43 (GJA1/CX43)，导致 GJA1 间隙连接斑块解聚和间隙连接活性抑制，这可能提供对氧化应激的保护作用 (by similar)。PKC gamma 还磷酸化 p53/TP53 并促进 DNA 损伤后 p53/TP53 依赖性凋亡。它参与时间限制性喂养期间大脑皮层昼夜节律时钟的相位重置，通过干扰 BMAL1 的泛素化来稳定这一核心时钟组分，从而抑制其降解，导致大脑皮层时钟的相位重置 (by similar)。此外，PKC gamma 磷酸化并激活 LRRK1，后者磷酸化参与细胞内运输的 RAB 蛋白。

Free

P05129

Predicted band size: 78 kDa; Observed band size: 78 kDa

Protein A affinity purified.

Cell membrane. Cytoplasm.

Non-conjugated

Unmodified

AB_3102517

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1/500-1/1000; IF: 1/50-1/200

• 
  Western blot analysis of extracts from A549(lane 2(20ug) and A549(lane 3(40ug) using PKC gamma Antibody (HY-P80435) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunocytochemistry analysis of HepG2 cells labeling PKC gamma with PKC gamma Antibody (HY-P80435) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PKC gamma Antibody (HY-P80435) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
• 
  Immunocytochemistry analysis of HepG2 cells labeling PKC gamma with PKC gamma Antibody (HY-P80435) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PKC gamma Antibody (HY-P80435) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

WB, ICC/IF

液体

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human PKC gamma.