1. 抗体
  2. 一抗
  3. 单克隆抗体 重组抗体
  4. PKC gamma 抗体 (YA125)

PKC gamma 抗体 (YA125)

目录号: HY-P80435
COA 抗体使用指南 技术支持

PKC gamma Antibody (YA125) 是一个兔来源、无偶联标记、抗 PKC gamma 的 IgG 单克隆抗体。

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规格 价格 是否有货 数量
10 μL ¥470 In-stock
50 μL ¥1220 In-stock
100 μL ¥2000 In-stock
250 μL   询价  

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  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验
  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

PKC gamma Antibody (YA125) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PKC gamma.

宿主

Rabbit

克隆性

Recombinant, Monoclonal

分子量
Predicted band size: 78 kDa;
Observed band size: 78 kDa
请注意:因蛋白存在修饰或聚体等情况,以实测为准,预测仅为参考。
反应种属
Human, Mouse, Rat
蛋白数据库
基因 ID
免疫原

A synthesized peptide derived from human PKC gamma aa654-697.

应用 & 推荐
稀释比例
应用 稀释比
WB
WB: 蛋白质免疫印迹
1:500-1:1000
ICC/IF
ICC/IF: 细胞免疫荧光
1:50-1:200
敏感性 Endogenous 纯度 Protein A affinity purified.
偶联 Non-conjugated 修饰 Unmodified
同型 IgG  
性状

液体

组分

Supplied in Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片
ALL WB ICC
  • Western blot analysis of extracts from A549(lane 2(20ug) and A549(lane 3(40ug) using PKC gamma Antibody (HY-P80435) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of HepG2 cells labeling PKC gamma with PKC gamma Antibody (HY-P80435) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PKC gamma Antibody (HY-P80435) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Immunocytochemistry analysis of HepG2 cells labeling PKC gamma with PKC gamma Antibody (HY-P80435) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with PKC gamma Antibody (HY-P80435) at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L(HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

背景
功能:钙激活的、磷脂和二酰甘油 (DAG) 依赖性的丝氨酸/苏氨酸蛋白激酶,在神经元细胞和眼组织中发挥多种作用,例如调节神经元受体 GRIA4/GLUR4 和 GRIN1/NMDAR1,调节与阿片类药物、疼痛和酒精敏感性相关的受体和神经元功能,介导缺血后的突触功能和细胞存活,以及抑制氧化应激后的间隙连接活性。该激酶结合并磷酸化 GRIA4/GLUR4 谷氨酸受体,并通过增加质膜相关 GRIA4 的表达来调节其功能。在用激动剂 3,5-二羟基苯基甘氨酸处理的原代小脑神经元中,该蛋白位于代谢型谷氨酸受体 GRM5/MGLUR5 的下游,并磷酸化 GRIN1/NMDAR1 受体,后者在突触可塑性、突触发生、兴奋性毒性、记忆获得和学习中起关键作用。该蛋白可能参与海马长时程增强 (LTP) 的调节,但可能并非突触可塑性过程所必需。该蛋白可能参与脊髓中μ型阿片受体介导的 G 蛋白激活的脱敏,并且可能对吗啡诱导的边缘前脑强化效应的发生和/或维持至关重要。该蛋白可能通过参与导致阿片受体磷酸化和降解的信号通路来调节μ型阿片受体的功能。此外,该蛋白还可能导致慢性吗啡诱导的伤害性感受处理改变。在神经性疼痛机制中发挥作用,并有助于维持外周炎症引起的异常性疼痛。通过介导乙醇的行为效应以及该药物对 GABA (A) 受体的作用,在乙醇的初始敏感性和耐受性中发挥重要作用。在脑缺血期间和之后,调节突触膜上的神经传递和细胞存活,并参与胰岛素诱导的坏死抑制,这是最大限度减少缺血性损伤的重要机制。在发育中的小脑中,浦肯野细胞神经支配过程中,需要消除多条攀缘纤维。在过氧化氢处理后,晶状体上皮细胞中该蛋白被激活,并磷酸化连接蛋白-43 (GJA1/CX43),导致 GJA1 间隙连接斑块解体和间隙连接活性抑制,这可能对氧化应激起到保护作用 (基于相似性)。磷酸化 p53/TP53,并促进 DNA 损伤后 p53/TP53 依赖性细胞凋亡。参与限时喂养期间大脑皮层生物钟的相位重置。通过干扰核心时钟组分 BMAL1 的泛素化,抑制其降解,从而稳定 BMAL1,导致大脑皮层生物钟的相位重置 (基于相似性)。磷酸化并激活 LRRK1,LRRK1 磷酸化参与细胞内运输的 RAB 蛋白 (PubMed:36040231)。
亚细胞定位:细胞质;细胞质,核周区;细胞膜;外周膜蛋白;突触,突触体;细胞突起,树突
表达水平:
组织特异性:在小脑皮层的浦肯野细胞中表达
亚基:与 CDCP1 相互作用。与 TP53INP1 和 p53/TP53 相互作用。
RRID
反应种属数据库
研究领域

Signal Transduction

中文名
PKC gamma 抗体
同用名
PRKCG; PKCG; Protein kinase C gamma type; PKC-gamma
文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
PKC gamma Antibody (YA125)
目录号:
HY-P80435
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