Rad51 抗体 (YA108)

Rad51 Antibody (YA108) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Rad51.

Rad51 在 homologous strand exchange 中发挥重要作用，这是通过 homologous recombination (HR) 进行 DNA 修复的关键步骤。它以 ATP 依赖的方式结合 single-stranded DNA，形成 nucleoprotein filaments，这对于 homology search 和 strand exchange 至关重要。Rad51 催化 homologous DNA partners 之间的 homology 识别和 strand exchange，从而在断裂的 DNA 和修复模板之间形成 joint molecule。在 replication stress 期间，Rad51 被招募以解决 stalled replication forks。此外，Rad51 是 PALB2-scaffolded HR 复合物的一部分，该复合物包含 BRCA2 和 RAD51C，被认为在通过 HR 进行 DNA 修复中发挥作用。在 oxidative stress 条件下，Rad51 与 RAD51C 和 XRCC3 一起参与调节 mitochondrial DNA 的拷贝数。Rad51 还参与 interstrand cross-link repair。

Free

Q06609

Predicted band size: 37 kDa; Observed band size: 37 kDa

Protein A affinity purified.

Chromosome, Cytoplasm, Cytoskeleton, Mitochondrion, Nucleus.

Non-conjugated

Unmodified

AB_3102412

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

WB: 1:1000; ICC/IF: 1:50-1:100; IHC-P: 1:50-1:1000; FC: 1:50-1:100; IP: Use at an assay dependent concentration.

• 
  Western blot analysis of extracts from Jurkat (lane 2(20μg)，NIH3T3 (lane 3(20μg) and HEK293(lane 4(20μg) using Rad51 (HY-P80297) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue using Rad51 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80297, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue using Rad51 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80297, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, ICC/IF, IHC-P, FC, IP

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human Rad51.AA range:1-50.