1. 抗体
  2. 一抗
  3. 单克隆抗体 重组抗体
  4. SUMO1 抗体 (YA047)

SUMO1 抗体 (YA047)

目录号: HY-P80905
COA 抗体使用指南 技术支持

SUMO1 Antibody (YA047) 是一个兔来源、无偶联标记、抗 SUMO1 的 IgG 单克隆抗体。

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规格 价格 是否有货 数量
10 μL ¥470 In-stock
50 μL ¥1220 In-stock
100 μL ¥2000 In-stock
250 μL   询价  

* Please select Quantity before adding items.

  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验
  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

SUMO1 Antibody (YA047) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to SUMO1.

宿主

Rabbit

克隆性

Recombinant, Monoclonal

分子量
Predicted band size: 12 kDa;
Observed band size: 80/12 kDa
请注意:因蛋白存在修饰或聚体等情况,以实测为准,预测仅为参考。
反应种属
Human, Mouse, Rat
蛋白数据库
基因 ID
免疫原

Synthetic peptide corresponding to Human Sumo 1.The exact sequence is proprietary to MCE.

应用 & 推荐
稀释比例
应用 稀释比
WB
WB: 蛋白质免疫印迹
1:500-1:1000
IHC-P
IHC-P: 石蜡切片样本的免疫组织化学
1:50-1:100
敏感性 Endogenous 纯度 affinity purified
偶联 Non-conjugated 修饰 Unmodified
同型 IgG  
性状

液体

组分

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片
ALL WB IHC-P
  • Western blot analysis of extracts from Hela(lane 2(20ug) , A431(lane 3(20ug) and NIH/3T3(lane 4(20ug) using SUMO1 Antibody (HY-P80905) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using SUMO1 Antibody (YA047). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80905, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using SUMO1 Antibody (YA047). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80905, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

背景
功能:SUMO1 是一种类泛素蛋白,可以以单体或赖氨酸连接的聚合物形式共价连接到蛋白质上。通过异肽键与底物共价连接需要先经 E1 复合物 SAE1-SAE2 激活,再与 E2 酶 UBE2I 连接,这一过程可被 PIAS1-4、RANBP2 或 CBX4 等 E3 连接酶促进。这种蛋白质赖氨酸残基的翻译后修饰在多种细胞过程中发挥着关键作用,例如核转运、DNA 复制和修复、有丝分裂以及信号转导。例如,SUMO1 参与将 RANGAP1 靶向核孔复合物蛋白 RANBP2。它还可以共价连接到电压门控钾通道 KCNB1,从而调节 KCNB1 的门控特性 (PubMed:19223394)。聚合的 SUMO1 链也容易发生多聚泛素化,这种多聚泛素化可作为蛋白酶体降解修饰蛋白的信号。也可能调控参与腭发育的基因网络。共价连接到 ZFHX3 (PubMed:24651376)
亚细胞定位:核膜;核斑;细胞质;细胞核,PML 体;细胞膜;细胞核
异构体 & 翻译后修饰:P63165 有两种异构体:P63165-1:11557 Da (预测值);P63165-2:8799 Da (预测值)。
前体形式经 SENP1 或 SENP2 切割是发挥功能所必需的;聚合 SUMO1 链经 RNF4 进行多聚泛素化修饰。
亚基:UBE2I 与 KCNB1 共价结合;UBE2I 可增强与 KCNB1 的交联,而 PIAS1 可减弱与 KCNB1 的交联 (PubMed:15931224, PubMed:19223394)。UBE2I 与 SAE2、RANBP2、PIAS1 和 PIAS2 相互作用。
RRID
反应种属数据库
研究领域

Cell Biology

中文名
SUMO1 抗体 (YA047)
同用名
SUMO1; SMT3C; SMT3H3; UBL1; OK/SW-cl.43; Small ubiquitin-related modifier 1; SUMO-1; GAP-modifying protein 1; GMP1; SMT3 homolog 3; Sentrin; Ubiquitin-homology domain protein PIC1; Ubiquitin-like protein SMT3C; Smt3C; Ubiquitin-like protein
文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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