2. Cell Cycle/DNA Damage
  3. CDK Wee1 Checkpoint Kinase (Chk)
  4. DHI1

DHI1 是一种抗白血病剂并且对 Jurkat (IC50 = 21.83 μM) 和 HL-60 (IC50 = 19.14 μM) 白血病细胞具有高选择性，对非癌细胞具有低毒性。DHI1 诱导 Jurkat 和 HL-60 白血病细胞 G2/M 细胞周期停滞和 HL-60 细胞的 S 期停滞，对细胞周期信号分子 Wee1, cyclin B1, cdc2 on Tyr15, Chk1 有明显影响。DHI1 通过破坏细胞骨架肌动蛋白丝来抑制 Jurkat 和 HL-60 细胞的迁移和侵袭性。DHI1 可用于研究血液恶性肿瘤。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

DHI1

DHI1 Chemical Structure

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 是否有货
50 mg   询价  
100 mg   询价  
250 mg   询价  

* Please select Quantity before adding items.

Customer Review

DHI1 相关抗体

Top Publications Citing Use of Products

查看 CDK 亚型特异性产品:

查看所有亚型
CDK1 CDK2 CDK3 CDK4 CDK5 CDK6 CDK7 CDK8 CDK9 CDK11 CDK12 CDK13 CDK14 CDK16 CDK19 CDC CLK Pho85 CDK10 CDK15 CDK17 CDK18 CDK20 PITSLRE

查看 Wee1 亚型特异性产品:

查看所有亚型
PKMYT1

查看 Checkpoint Kinase (Chk) 亚型特异性产品:

查看所有亚型
Chk1 Chk2 BUBR1

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

DHI1 is an anti-leukemia agent with high selectivity for Jurkat (IC50 = 21.83 μM) and HL-60 (IC50 = 19.14 μM) leukemia cells and has low toxicity to non-cancerous cells. DHI1 can induce G2/M phase cell arrest in Jurkat and HL-60 leukemia cells, as well as S phase arrest in HL-60 cells, and has significant effects on cell cycle signaling molecules Wee1, cyclin B1, cdc2 on Tyr15, and Chk1. DHI1 inhibits the migration and invasion of Jurkat and HL-60 cells by disrupting cytoskeletal actin filaments. DHI1 can be used to study hematological malignancies[1].

IC50 & Target[1]

Chk1

 

体外研究
(In Vitro)

DHI1 (Compound 4a) (3-100 μM，24-72 小时) 对 Jurkat 和 HL-60 细胞表现出抑制作用，IC50 分别为 21.83 和 19.14 μM，但对 HCT-116、HeLa、MCF-7、U87、Hep G2、A549、A2780、BJ-5ta 和 MCF-10A 细胞的抑制作用较弱[1]。
DHI1 (19.14-21.83 μM，24-72 小时) 在 Jurkat 和 HL-60 细胞中诱导细胞周期停滞于 G2/M 期，并影响与细胞周期相关的信号蛋白[1]。
DHI1 (10-40 μM，3 小时) 降低 Jurkat 和 HL-60 白血病细胞的趋化性和侵袭性[1]。
DHI1 (19.14-21.83 μM，24-72 小时) 在 Jurkat 和 HL-60 白血病细胞中可诱导 F- 肌动蛋白结构破坏、紊乱、损伤，以及核碎裂和膜起泡，影响细胞骨架[1]。
DHI1 (3.125-100 μM，24 小时) 可增强 PBMC 细胞活力[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

DHI1 相关抗体:

Cell Cycle Analysis[1]

Cell Line: Jurkat cells
Concentration: 21.83 μM
Incubation Time: 24 h, 48 h, 72 h
Result: Induced cell cycle arrest at G2/M phase at 24 h and increases sub-G0/G1 fraction. Reduced phosphorylation of Wee1 (Ser642) , cyclin B1 (Ser133), cdc2 on Tyr15, human retinoblastoma protein (Rb) .
Increased phosphorylation of Chk1 (Ser345), but reduced phosphorylation of Chk1 (Ser345) after 72 hours.
Increased the level of p21 at 24 and 48 h.

Cell Cycle Analysis[1]

Cell Line: HL-60 cells
Concentration: 19.14 μM
Incubation Time: 24 h, 48 h, 72 h
Result: Induced cell cycle arrest at G2/M phase after 24 h, blocked S phase, and increased sub-G0/G1 peak of DNA fragmentation.
Reduced phosphorylation of Wee1 (Ser642), cdc2 on Tyr15, human retinoblastoma protein (Rb), increased phosphorylation of Chk1 (Ser345).
Increased the level of p21 at 24 h, but decrease p21 at 48-72 h.

Cell Migration Assay [1]

Cell Line: Jurkat and HL-60 cells
Concentration: 10, 20, and 40 μM
Incubation Time: 3 h
Result: Decreased relative cell chemotaxis by 31.1 and 45.7% at 40 μM, reduced HL-60 cell invasion by 17.7% at 20 μM.
Induced concentration-dependent effects on the invasiveness/motility, reduced cell invasion by 39.7 and 57.8% at 40 μM, reduced HL-60 cell invasion by 33.6% at 20 μM.
分子量

355.23

Formula

C18H15BrN2O
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

DHI1 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DHI1DHI 1DHI-1CDKWee1Checkpoint Kinase (Chk)Cyclin dependent kinaseHematological malignanciesJurkat cellsHL-60 cellsInhibitorinhibitorinhibit

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称：

  

* 需求量：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Bulk Inquiry

Inquiry Information

产品名称：
DHI1
目录号：
HY-175261
需求量：

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

   产品名称：

  

* Lot #：

* 客户姓名：

 

* Email：

   电话：

 

* 部门：

* 公司或机构名称：

 

   留言给我们：

Request for HNMR Report

We have received your request and will respond to you as soon as possible.

嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z