1. MCE Kits
  2. Molecular Biology
  3. RT-PCR
  4. Reverse transcription PCR
  5. RT Master Mix for qPCR II (gDNA digester plus)

RT Master Mix for qPCR II (gDNA digester plus) 

反转录试剂盒 II (预混 gDNA 消化酶)

Cat. No.: HY-K0511A
Manual SDS

RT Master Mix for qPCR II (gDNA digester plus) 反转录即用型预混液,采用升级款反转录酶,反应更高效、快速,适用于高 GC 含量、复杂二级结构、少量 RNA 模板等。试剂盒配有 gDNA digester 可去除 RNA 模板中的基因组 DNA 污染。

RT Master Mix for qPCR II (gDNA digester plus)

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
100 rxns ¥1480 In-stock
500 rxns ¥6600 In-stock

* Please select Quantity before adding items.

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  • Description

  • Storage

  • Protocol

  • Components

  • Documentation

Description
& Advantages

MCE RT Master Mix is a convenient, ready-to-use formulation for reverse transcription. This kit contains 5x gDNA digester Mix which can eliminate gDNA contaminations in RNA samples. 4× Super RT Mix contains all the reagents necessary for first-strand cDNA synthesis. In addition, the increased heat resistance of the advanced reverse transcriptase allows reverse transcription to be performed at a higher temperature, increasing the efficiency and specificity of cDNA synthesis.

Upon completion of the first-strand cDNA synthesis, the cDNA product can be directly applied as a template in a standard PCR and qPCR. MCE SYBR Green qPCR Master Mix (HY-K0501) is highly recommended for detection of the expression levels of interested genes.

 

Features:

1. Synthesize cDNA rapidly and specifically.

2. Super-fast gDNA removal in 2 minutes.

3. Optimized buffer enhances sensitive and reliable cDNA synthesis.

4. Super-efficient reaction even with low template amounts (total RNA: 50 pg -5 μg in a 20 μL reaction).

5. The kit provides both Oligo dT Primer and Random Primer.

Publications
Storage

Stored at -20°C, and is stable for up to 18 months.

Avoid repetitive freeze-thaw cycles.

Protocol

1   Thaw RNA templates, gDNA digester, 5× gDNA digester Buffer and the 4× Super RT Mix on ice. Mix solutions gently but thoroughly.

2   Prepare the following reaction mixture in a PCR tube on ice. Mix thoroughly, and incubate at 42ºC for 2 minutes.

Components Quantity
5× gDNA digester Buffer 3 μL
Total RNA / mRNA 50 pg-5 μg / 50 pg-500 ng
RNase-Free H2O To 15 μL

3   Add 5 μL of 4× Super RT Mix (gDNA digester inhibitor contained) to the mixture from Step 2 (15 μL). Mix the components well and collect by brief centrifugation. Incubate the mixture in a PCR instrument or water bath in the procedure as follows:

Temperature Time
25ºC 5 mins
55ºC 15 mins
85ºC 2 mins

Note:

a.  For GC rich or structurally complex RNA templates, increasing the RT incubation temperature up to 60ºC may improve the yields of cDNA.

b.  Stop the reaction by heating at 85°C for 2 minutes followed by chilling on ice.

4   The newly synthesized first-strand cDNA is ready for immediate downstream applications or for long-term storage at -20ºC.

Components
Contents HY-K0511A-100 rxns HY-K0511A-500 rxns
5× gDNA digester Mix 300 μL 300 μL × 5
4× Super RT Mix 500 μL 500 μL × 5
RNase-Free H2O 1 mL × 2 1 mL × 10
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
反转录试剂盒 II (预混 gDNA 消化酶)
目录号:
HY-K0511A
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