1. Academic Validation
  2. 2,2'-Bispyridyl disulfide rapidly induces intramolecular disulfide bonds in peptides

2,2'-Bispyridyl disulfide rapidly induces intramolecular disulfide bonds in peptides

  • Peptides. 1999;20(7):881-4. doi: 10.1016/s0196-9781(99)00076-5.
K Maruyama 1 H Nagasawa A Suzuki
Affiliations

Affiliation

  • 1 Molecular Medicine Laboratories, Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co., Ltd., Tsukuba, Ibaraki, Japan. maruyama@yamanouchi.co.jp
Abstract

A linear peptide containing two reduced cysteine residues can be rapidly converted to its oxidized cyclic form containing an intramolecular disulfide bond by adding an excess of 2,2'-bispyridyl disulfide (2,2'-dipyridyl disulfide or 2,2'-dithiodipyridine) to conventional buffer solutions. The reactants and products are easily separated by reverse-phase chromatography. This reaction will find wide application in forming intramolecular disulfide bonds because of its selectivity for free sulfhydryl groups, quickness, safety, and applicability under acidic conditions.

Figures
Products