1. Academic Validation
  2. Amperometric sensing of norepinephrine at picomolar concentrations using screen printed, high surface area mesoporous carbon

Amperometric sensing of norepinephrine at picomolar concentrations using screen printed, high surface area mesoporous carbon

  • Anal Chim Acta. 2013 Jul 25;788:32-8. doi: 10.1016/j.aca.2013.06.019.
Mingzhi Dai 1 Brittney Haselwood Bryan D Vogt Jeffrey T La Belle
Affiliations

Affiliation

  • 1 Chemical Engineering Program, Arizona State University, Tempe, AZ 85287, United States. mingzhi.dai@asu.edu
Abstract

Norepinephrine (NE) is detected amperometrically using the Enzyme Phenylethanolamine N-methyl transferase and cofactor S-(5'-Adenosyl)-l-methionine chloride dihydrochloride with disposable screen printed mesoporous carbon electrodes. The role of internal surface area and pore size of the mesoporous carbon is systematically examined using soft-templated, mesoporous silica-carbon powders with highly microporous walls obtained from etching of the silica to produce powders with surface areas ranging from 671-2339 m(2)g(-1). As the surface area increases, the sensitivity of the biosensor at very low NE concentrations (0-500 pg mL(-1)) in phosphate buffered saline (PBS) increases just as the current signal increases with respect to the NE concentration of 81-1581 μA mL ng(-1) cm(-2) for the mesoporous carbons. The best performing electrode provides similar sensitivity in whole rabbit blood in comparison to PBS despite no membrane layer to filter the non-desired reactants; the small (<5 nm) pore size and large internal surface area acts to minimize non-specific events that decrease sensitivity.

Keywords

Electrochemistry; Mesoporous carbon; Norepinephrine; Porous materials; Self-assembly; Sensor.

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