1. Academic Validation
  2. Analysis, physiological and clinical significance of 12-HETE: a neglected platelet-derived 12-lipoxygenase product

Analysis, physiological and clinical significance of 12-HETE: a neglected platelet-derived 12-lipoxygenase product

  • J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Aug 1;964:26-40. doi: 10.1016/j.jchromb.2014.03.015.
Benedetta Porro 1 Paola Songia 1 Isabella Squellerio 1 Elena Tremoli 2 Viviana Cavalca 3
Affiliations

Affiliations

  • 1 Centro Cardiologico Monzino-IRCCS, Milan, Italy.
  • 2 Centro Cardiologico Monzino-IRCCS, Milan, Italy; Università degli Studi di Milano, Dipartimento di Scienze Farmacologiche e Biomolecolari, Milan, Italy.
  • 3 Centro Cardiologico Monzino-IRCCS, Milan, Italy; Dipartimento di Scienze Cliniche e di Comunità, Milan, Italy. Electronic address: viviana.cavalca@unimi.it.
Abstract

While the importance of cyclooxygenase (COX) in platelet function has been amply elucidated, the identification of the role of 12-lipoxygenase (12-LOX) and of its stable metabolite, 12-hydroxyeicosatretraenoic acid (12-HETE), has not been clarified as yet. Many studies have analysed the implications of 12-LOX products in different pathological disorders but the information obtained from these works is controversial. Several analytical methods have been developed over the years to simultaneously detect eicosanoids, and specifically 12-HETE, in different biological matrices, essentially enzyme-linked immunosorbent assays (ELISA), radioimmunoassays (RIA), high performance liquid chromatography (HPLC) and mass spectrometry coupled with both gas and liquid chromatography methods (GC- and LC-MS). This review is aimed at summarizing the up to now known physiological and clinical features of 12-HETE together with the analytical methods used for its determination, focusing on the critical issues regarding its measurement.

Keywords

12-HETE; 12-LOX clinical implication; Analytical method; Arachidonic acid metabolism; Platelet.

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