2. Academic Validation
  3. Piperlonguminine is neuroprotective in experimental rat stroke

Piperlonguminine is neuroprotective in experimental rat stroke

  • Int Immunopharmacol. 2014 Dec;23(2):447-51. doi: 10.1016/j.intimp.2014.09.016.
Tiansong Yang 1,  Shixiao Sun 2,  Tiegang Wang 3,  Xin Tong 4,  Junhui Bi 5,  Yulin Wang 1,  Zhongren Sun 6
Affiliations

Affiliations

  • 1 First Affiliated Hospital, Heilongjiang University of Chinese Medicine, PR China.
  • 2 Department of Physiology, Heilongjiang University of Chinese Medicine, PR China.
  • 3 The First Affiliated Hospital, Harbin Medical University, Harbin, PR China.
  • 4 Atlantic Institute of Oriental Medicine (ATOM), FL, USA.
  • 5 Department of Formulas of Chinese Medicine, Heilongjiang University of Chinese Medicine, PR China.
  • 6 First Affiliated Hospital, Heilongjiang University of Chinese Medicine, PR China; Department of Acupuncture, Heilongjiang University of Chinese Medicine, PR China. Electronic address: sunzhong_ren@163.com.
PMID: 25257731 DOI: 10.1016/j.intimp.2014.09.016
Abstract

Inflammatory damage plays an important role in cerebral ischemic pathogenesis and may represent a target for treatment. Piperlonguminine (PE) has been proved to have anti-inflammatory actions. In this study, we investigated the effects of PE on cultured neuronal cell line, SH-SY5Y in vitro and experimental rat ischemic stroke in vivo. For oxygen-glucose deprivation (OGD) and tumor necrosis factor-α (TNF-α) stimulated SH-SY5Y cell line in vitro, SH-SY5Y cells were incubated with PE. In vivo, rats were subjected to middle cerebral artery occlusion (MACO) for 1h, followed by reperfusion for 23 h. The results of this study showed that treatment of SH-SY5Y cells with PE reduced the OGD-induced cytotoxicity and Apoptosis and blocked TNF-α-induced activation of NF-κB and MAPK. Intraperitoneal injection of PE (2.4 mg/kg) produced a significant neuroprotective potential in rats with cerebral ischemia. PE attenuated neurological deficit scores, brain infarct volume and brain water content in rats, and inhibited activation of NF-κB and MAPK. These data show that PE protects the brain against ischemic cerebral injury via alleviating blood-brain barrier (BBB) breakdown, which may be mediated via inhibiting NF-κB and MAPK signaling pathways.

Keywords

Experimental stroke; Piperlonguminine; Rats; Signaling pathways.

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