2. Academic Validation
  3. Bioconversion of rebaudioside I from rebaudioside A

Bioconversion of rebaudioside I from rebaudioside A

  • Molecules. 2014 Oct 28;19(11):17345-55. doi: 10.3390/molecules191117345.
Indra Prakash 1,  Cynthia Bunders 2,  Krishna P Devkota 3,  Romila D Charan 4,  Catherine Ramirez 5,  Tara M Snyder 6,  Christopher Priedemann 7,  Avetik Markosyan 8,  Cyrille Jarrin 9,  Robert Ter Halle 10
Affiliations

Affiliations

  • 1 The Coca-Cola Company, Atlanta, GA 30313, USA. iprakash@coca-cola.com.
  • 2 The Coca-Cola Company, Atlanta, GA 30313, USA. cbunders@coca-cola.com.
  • 3 AMRI-Albany, Analytical Development, Albany, NY 12212, USA. Krishna.Devkota@amriglobal.com.
  • 4 AMRI-Albany, Analytical Development, Albany, NY 12212, USA. Romila.Charan@amriglobal.com.
  • 5 AMRI-Albany, Analytical Development, Albany, NY 12212, USA. Catherine.Ramirez@amriglobal.com.
  • 6 AMRI-Albany, Analytical Development, Albany, NY 12212, USA. Tara.Snyder@amriglobal.com.
  • 7 AMRI-Albany, Analytical Development, Albany, NY 12212, USA. Christopher.Priedemann@amriglobal.com.
  • 8 PureCircle Limited, Lengkuk Teknologi, Bandar Enstek 71760, Malaysia. avetik@purecircle.com.
  • 9 Libragen SA, 3 Rue des Satellites, Toulouse 31400, France. c.jarrin@libragen.com.
  • 10 Libragen SA, 3 Rue des Satellites, Toulouse 31400, France. r.terhalle@libragen.com.
PMID: 25353385 DOI: 10.3390/molecules191117345
Abstract

To supply the increasing demand of natural high potency Sweeteners to reduce the calories in food and beverages, we have looked to steviol glycosides. In this work we report the bioconversion of rebaudioside A to rebaudioside I using a glucosyltransferase Enzyme. This bioconversion reaction adds one sugar unit with a 1→3 linkage. We utilized 1D and 2D NMR spectroscopy (1H, 13C, COSY, HSQC-DEPT, HMBC, 1D TOCSY and NOESY) and mass spectral data to fully characterize rebaudioside I.

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