2. Academic Validation
  3. The Mechanism of Action of Lysobactin

The Mechanism of Action of Lysobactin

  • J Am Chem Soc. 2016 Jan 13;138(1):100-3. doi: 10.1021/jacs.5b11807.
Wonsik Lee 1,  Kaitlin Schaefer 1 2,  Yuan Qiao 1 2,  Veerasak Srisuknimit 2,  Heinrich Steinmetz 3,  Rolf Müller 3,  Daniel Kahne 2,  Suzanne Walker 1
Affiliations

Affiliations

  • 1 Department of Microbiology and Immunology, Harvard Medical School , Boston, Massachusetts 02115, United States.
  • 2 Department of Chemistry and Chemical Biology, Harvard University , Cambridge, Massachusetts 02138, United States.
  • 3 Department of Microbial Natural Products, Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Centre for Infection Research (HZI), and Pharmaceutical Biotechnology, Saarland University , Campus E8.1, 66123 Saarbrücken, Germany.
PMID: 26683668 DOI: 10.1021/jacs.5b11807
Abstract

Lysobactin, also known as katanosin B, is a potent Antibiotic with in vivo efficacy against Staphylococcus aureus and Streptococcus pneumoniae. It was previously shown to inhibit peptidoglycan (PG) biosynthesis, but its molecular mechanism of action has not been established. Using Enzyme inhibition assays, we show that lysobactin forms 1:1 complexes with Lipid I, Lipid II, and Lipid II(A)(WTA), substrates in the PG and wall teichoic acid (WTA) biosynthetic pathways. Therefore, lysobactin, like ramoplanin and teixobactin, recognizes the reducing end of lipid-linked cell wall precursors. We show that despite its ability to bind precursors from different pathways, lysobactin's cellular mechanism of killing is due exclusively to Lipid II binding, which causes septal defects and catastrophic cell envelope damage.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z