2. Academic Validation
  3. Stability and properties of Z-DNA containing artificial nucleobase 2'-O-methyl-8-methyl guanosine

Stability and properties of Z-DNA containing artificial nucleobase 2'-O-methyl-8-methyl guanosine

  • Bioorg Med Chem. 2019 Jan 15;27(2):364-369. doi: 10.1016/j.bmc.2018.12.005.
Thananjeyan Balasubramaniyam 1,  Takumi Ishizuka 1,  Yan Xu 2
Affiliations

Affiliations

  • 1 Division of Chemistry, Department of Medical Sciences, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyo-take, Miyazaki 889-1692, Japan.
  • 2 Division of Chemistry, Department of Medical Sciences, Faculty of Medicine, University of Miyazaki, 5200 Kihara, Kiyo-take, Miyazaki 889-1692, Japan. Electronic address: xuyan@med.miyazaki-u.ac.jp.
PMID: 30545733 DOI: 10.1016/j.bmc.2018.12.005
Abstract

We synthesized several DNA Oligonucleotides containing one or several 2'-O-methyl-8-methyl guanosine (m8Gm) and demonstrated that these Oligonucleotides not only stabilize the Z-DNA with a wide range of sequences under low salt conditions but also possess high thermal stability. Using artificial nucleobase-containing Oligonucleotides, we studied the interaction of the Zα domain with Z-DNA. Furthermore, we showed that the m8Gm-contained Oligonucleotides allow to study the photochemical reaction of Z-DNA.

Keywords

Artificial nucleobase; Circular dichroism; Interaction of protein and Z-DNA; Photochemical reaction; Z-DNA oligonucleotide.

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