1. Academic Validation
  2. Wilms' tumour 1 (WT1) negatively regulates the expression of connexin 43 via a non-canonical Wnt signalling pathway in cultured bovine Sertoli cells

Wilms' tumour 1 (WT1) negatively regulates the expression of connexin 43 via a non-canonical Wnt signalling pathway in cultured bovine Sertoli cells

  • Reprod Fertil Dev. 2020 Mar;32(5):522-530. doi: 10.1071/RD19088.
Xue Wang 1 Ziming Wang 1 S O Adeniran 1 Fushuo Huang 1 Mingjun Ma 1 Han Zhang 1 Xiaoyu Li 1 Peng Zheng 1 Guixue Zhang 2
Affiliations

Affiliations

  • 1 Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Northeast Agricultural University, No. 600 Changjiang Street, Xiangfang District, 150030 Harbin, PR China.
  • 2 Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Northeast Agricultural University, No. 600 Changjiang Street, Xiangfang District, 150030 Harbin, PR China; and Corresponding author. Email: Gxzhang@neau.edu.cn.
Abstract

The Gap Junction Protein connexin (Cx) 43 between adjacent Sertoli cells (SCs) is the main testicular factor regulating the growth and development of SCs, and plays a vital role in controlling cell differentiation and maturation. However, the endogenous testicular factors that regulate Cx43 and the downstream signalling pathways that mediate Cx43-dependent SC differentiation are unclear. In this study, high-purity SCs were isolated from newborn calves' testes by differential adherence. The SCs were then cultured invitro and treated with short interference RNA to knockdown endogenous Wilms' tumour 1 (WT1). In WT1-knockdown SCs, Cx43 expression was upregulated. To elucidate the intracellular signalling mechanism of Cx43 in the differentiation and maturation of immature SCs, SCs were treated simultaneously with non-canonical Wnt signalling inhibitors CCG-1423 and GO-6983; in these SCs, Cx43 expression was upregulated. Together, these data indicate that WT1 negatively regulates the expression of Cx43 produced from SCs via a non-canonical Wnt signalling pathway in cultured bovine SCs.

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