1. Academic Validation
  2. AMP-activated kinase regulates porcine reproductive and respiratory syndrome virus infection in vitro

AMP-activated kinase regulates porcine reproductive and respiratory syndrome virus infection in vitro

  • Virus Genes. 2022 Apr;58(2):133-142. doi: 10.1007/s11262-022-01888-7.
Jianyu Fang 1 Hongkui Wang 2 Limin Lang 3 Haili Li 3 Shaoyu Li 3 Keling Wang 3
Affiliations

Affiliations

  • 1 Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China. fangjianyu2006@126.com.
  • 2 Livestock Product Quality Inspection Institute, Henan University of Animal Husbandry and Economy, Zhengzhou, 450046, China.
  • 3 Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.
Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) is an economically important pathogen in the pig industry worldwide. Many viruses manipulate their cellular metabolism to replicate themselves and cause Infection. A conserved cellular energy sensor, 5'-AMP-activated protein kinase (AMPK), maintains cellular energy homeostasis. We found that PRRSV Infection caused significant AMPK activation in a time-dependent manner via the ROS-calcium/calmodulin-dependent protein kinase-2 pathway. RNA interference-mediated AMPK knockdown could increase PRRSV replication in MARC-145 cells, suggesting that AMPK contributed to PRRSV Infection regulation. Moreover, investigation of the effect of AMPK activity on PRRSV replication showed that PRRSV replication could be suppressed by the pharmacological agonists 5-aminoimidazole-4-carboxamide-1-β-D-ribofuranoside and A769662. Conversely, an AMPK Inhibitor, compound C, markedly enhanced PRRSV Infection. Furthermore, the AMPK agonist A769662 was found to exert no effect on PRRSV entry, assembly, and release, suggesting that A769662 may hinder the PRRSV genome replication in MARC-145 cells. In conclusion, AMPK may be a promising Antiviral drug target against PRRSV Infection.

Keywords

AMP-activated protein kinase; Phosphorylation; Porcine reproductive and respiratory syndrome; Virus replication and infection.

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