2. Academic Validation
  3. Stress conditions induced circRNAs profile of extracellular vesicles in brain microvascular endothelial cells

Stress conditions induced circRNAs profile of extracellular vesicles in brain microvascular endothelial cells

  • Metab Brain Dis. 2022 Aug;37(6):1977-1987. doi: 10.1007/s11011-022-01025-1.
Xiao-Li Min 1,  Hecun Zou 2,  Jianghong Yan 2,  Qiang Lyu 3,  Xiang He 4,  Fei-Fei Shang 5
Affiliations

Affiliations

  • 1 Department of Cerebrovascular Diseases, The Second Affiliated Hospital of Kunming Medical University, Kunming, 650101, Yunnan Province, People's Republic of China.
  • 2 Institute of Life Science, Chongqing Medical University, 400016, Chongqing, People's Republic of China.
  • 3 Department of Anesthesiology, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, 610072, Sichuan Province, People's Republic of China.
  • 4 Department of Anesthesiology, Guizhou Provincial People's Hospital, Guiyang, 550002, Guizhou Province, People's Republic of China.
  • 5 Institute of Life Science, Chongqing Medical University, 400016, Chongqing, People's Republic of China. sff_phoenix@cqmu.edu.cn.
PMID: 35699856 DOI: 10.1007/s11011-022-01025-1
Abstract

Cerebral ischemia causes hypoxic injury and inflammation, and brain microvascular endothelial cells (BMVECs) dysfunction is an initial stage of blood-brain barrier disruption. Endothelial cells secrete extracellular vesicles (EVs) that are involved in intercellular signal transduction. EVs contain a variety of RNAs, proteins, and metabolites. Circular RNA (circRNA) is a member of the non-coding RNA. The expression profile and potential function of circRNAs in BMVECs are unknown. Here, human BMVECs have undergone hypoxia or TNF-α induction, and the changes in circRNAs were measured by RNA sequencing. A total of 70 circRNAs showed differential expression, including 43 previously unrecorded circRNAs and 27 recorded circRNAs. Since astrocyte end-feet encircle endothelial cells, they are considered the main targets of the EVs from BMVEC. The miRNA sequence data and bioinformatics were used to predict the circRNA-miRNA-mRNA networks in astrocytes. The gene ontology (GO) analysis showed the main downstream targets of circRNAs are DNA transcription regulation and protein kinase-related signaling pathways. These results suggest that altering circRNAs may be a potential therapeutic target for cerebral ischemia induced hypoxic injury and inflammation.

Keywords

Cerebral ischemia; Extracellular vesicles; Human brain microvascular endothelial cells; circRNAs.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z