1. Academic Validation
  2. Adipose tissue-derived Muse cells promote autophagy and oxidative stress tolerance in human epidermal melanocytes

Adipose tissue-derived Muse cells promote autophagy and oxidative stress tolerance in human epidermal melanocytes

  • Cell Tissue Bank. 2022 Aug 20. doi: 10.1007/s10561-022-10031-7.
Shengyi Wang  # 1 2 Peng Wang  # 3 Ruzhi Zhang 4
Affiliations

Affiliations

  • 1 Department of Dermatology, The Third Affiliated Hospital of Soochow University, Changzhou, China.
  • 2 Xuzhou Central Hospital, Xuzhou, Jiangsu, China.
  • 3 Department of Intensive Care Unit, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, China.
  • 4 Department of Dermatology, The Third Affiliated Hospital of Soochow University, Changzhou, China. zhangruzhi628@163.com.
  • # Contributed equally.
Abstract

To investigate the effect of human adipose tissue-derived multilineage-differentiating stress-enduring (Muse) cells on the oxidative stress injury of human epidermal melanocytes (HEMs) in vitro. HEMs were treated with H2O2 to establish an oxidative stress injury model and then were co-cultured with adipose tissue-derived Muse cells. Immunohistochemistry, flow cytometry and Western blotting were used to assess changes in Autophagy flux, Apoptosis, expression of melanin synthesis related proteins and proliferation of melanocytes. Our findings demonstrate that co-culture with Muse cells significantly increased the tolerance of HEMs to oxidative stress, enhanced Autophagy flux and reduced Apoptosis. The expression of proteins related to the formation of melanin increased as did cell proliferation. Treatment with the Autophagy Inhibitor, 3-methyladenine (3MA), partially counteracted the improvement of oxidative stress tolerance in melanocytes elicited by co-culture with Muse cells. Muse cells promote Autophagy and oxidative stress tolerance of melanocytes.

Keywords

Human epidermal melanocytes (HEMs); Multi-lineage differentiating stress-enduring cells (Muse cells); Reactive oxygen species (ROS).

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