2. Academic Validation
  3. Amoxetamide A, a new anoikis inducer, produced by combined-culture of Amycolatopsis sp. and Tsukamurella pulmonis

Amoxetamide A, a new anoikis inducer, produced by combined-culture of Amycolatopsis sp. and Tsukamurella pulmonis

  • J Antibiot (Tokyo). 2023 Oct 30. doi: 10.1038/s41429-023-00668-1.
Chengqian Pan # 1 2,  Hiroaki Ikeda # 1,  Mayuri Minote 1,  Tensei Tokuda 1,  Takefumi Kuranaga 1,  Tohru Taniguchi 3,  Naoya Shinzato 4,  Hiroyasu Onaka 5,  Hideaki Kakeya 6
Affiliations

Affiliations

  • 1 Department of System Chemotherapy and Molecular Sciences, Division of Medicinal Frontier Sciences, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, 606-8501, Japan.
  • 2 School of Pharmacy, Jiangsu University, Zhenjiang, 212013, China.
  • 3 Faculty of Advanced Life Science, Hokkaido University, Sapporo, 001-0021, Japan.
  • 4 Tropical Biosphere Research Center, University of the Ryukyus, Okinawa, 903-0213, Japan.
  • 5 Department of Life Science, Faculty of Science, Gakushuin University, Tokyo, 171-8588, Japan.
  • 6 Department of System Chemotherapy and Molecular Sciences, Division of Medicinal Frontier Sciences, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, 606-8501, Japan. scseigyo-hisyo@pharm.kyoto-u.ac.jp.
  • # Contributed equally.
PMID: 37903880 DOI: 10.1038/s41429-023-00668-1
Abstract

Cancer cells including colorectal Cancer cells are resistant to anoikis, an anchorage-independent programmed death, which enables metastasis and subsequent survival in a new tumor microenvironment. In this study, we identified a new anoikis inducer, amoxetamide A (1) with a β-lactone moiety, that was produced by combined-culture of Amycolatopsis sp. 26-4 and mycolic acid-containing bacteria (MACB) Tsukamurella pulmonis TP-B0596. The structure of 1 including the stereochemistry of C8 was determined by MS and NMR spectroscopy and modified Mosher's method, and the absolute configurations of C11 and C12 were suggested as 11R and 12S, respectively, by GIAO NMR calculations. Amoxetamide A (1) exhibited anoikis-inducing activity in human colorectal Cancer HT-29 cells in anchorage-independent culture conditions.

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