1. Academic Validation
  2. Dibenzylideneacetone Overcomes Botrytis cinerea Infection in Cherry Tomatoes by Inhibiting Chitinase Activity

Dibenzylideneacetone Overcomes Botrytis cinerea Infection in Cherry Tomatoes by Inhibiting Chitinase Activity

  • J Agric Food Chem. 2023 Nov 1. doi: 10.1021/acs.jafc.3c05695.
Xiaodi Niu 1 Ziyou Wang 1 Chenyang Wang 1 Hongsu Wang 1
Affiliations

Affiliation

  • 1 College of Food Science and Engineering, Jilin University, Changchun 130062, P. R. China.
Abstract

Chitinase, a crucial component of the Fungal cell wall and septa, plays an important role in Fungal germination by hydrolyzing chitin to provide carbon and energy for Fungal growth and reproduction. In this study, we initially screened dibenzylideneacetone (DBA), a small molecule with inhibitory activity against Botrytis cinerea Chitinase, exhibiting an IC50 of 13.10 μg/mL. By constructing a three-dimensional (3D) model of the B. cinerea Chitinase and utilizing computational biology approaches, we found DBA bound to the active site pocket and formed strong π-π interactions and hydrophobic interactions with Chitinase, indicative of its competitive inhibitory mode. Site-directed mutagenesis also revealed that TRP-382, TRP-135, and ALA-215 were key amino acid residues involved in DBA binding. Subsequent Antifungal assays showed that DBA had an MIC of 32 μg/mL against B. cinerea and EC50 values of 16.29 and 14.64 μg/mL in inhibiting mycelial growth and spore germination, respectively. Importantly, in vivo experiments demonstrated that DBA treatment significantly extended the shelf life of cherry tomatoes by 2-fold. Therefore, DBA represents a promising Antifungal agent for fruit preservation applications.

Keywords

Botrytis cinerea; chitinase; fruit preservation; molecule modeling.

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