2. Metabolic Enzyme/Protease
  3. HIF/HIF Prolyl-Hydroxylase
  4. Naphthofluorescein

Naphthofluorescein  (Synonyms: Naphthafluorescein)

目录号: HY-124473 纯度: 99.52%
COA 产品使用指南

摩尔计算器

Naphthofluorescein 抑制 HIF-1 和 Mint3 之间的相互作用。Naphthofluorescein 抑制 Mint3 依赖性 HIF-1 活性以及癌细胞和巨噬细胞的糖酵解活动，同时不引起体外细胞毒性和体内副作用。Naphthofluorescein 也是一种荧光 pH 敏感探针，可用于功能性切伦科夫成像。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

Naphthofluorescein Chemical Structure

Naphthofluorescein Chemical Structure

CAS No. : 61419-02-1

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
5 mg ￥200
In-stock
10 mg ￥300
In-stock
25 mg ￥540
In-stock
50 mg ￥756
In-stock
100 mg ￥1000
In-stock
200 mg   询价  
500 mg   询价  

* Please select Quantity before adding items.

Customer Review

Naphthofluorescein 相关抗体

Top Publications Citing Use of Products

查看 HIF/HIF Prolyl-Hydroxylase 亚型特异性产品:

查看所有亚型
HIF-1α HIF

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Naphthofluorescein inhibits the interaction between HIF-1 and Mint3. Naphthofluorescein suppresses Mint3-dependent HIF-1 activity and glycolysis in cancer cells and macrophages without cytotoxicity in vitro and adverse effect in vivo[1]. Naphthofluorescein is also a fluorescent pH-sensitive probe that can be used for functional Cerenkov imaging[2].

IC50 & Target

HIF-1[1]
体外研究
(In Vitro)

Naphthofluorescein (compound 19) (0-10 μM; 24 hours) suppresses the HIF-1 reporter activity in a concentration-dependent manner[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Naphthofluorescein 相关抗体:

Cell Viability Assay[1]

Cell Line: HT1080 cells
Concentration: 0-10 μM
Incubation Time: 24 hours
Result: Significantly suppressed the HIF-1 reporter activity in a concentration-dependent manner.
体内研究
(In Vivo)

Naphthofluorescein (compound 19) (100 mg/kg; i.p.; once daily for 5 consecutive days followed by 2 days off for 2 weeks)does not show weight loss or apparent histological abnormalities in the lung, liver, and kidney, or cause cause severe adverse effects for at least 2 weeks in mice[1].
Naphthofluorescein (compound 19) (100 mg/kg; i.p.; once daily for 5 consecutive days followed by 2 days off for 2 weeks) strikingly suppresses the tumour growth of subcutaneously injected E0771 cells and significantly attenuates tumour growth of MDA-MB-231 and AsPC-1 cells in immunodeficient mice. In turn, naphthofluorescein does not attenuate tumour growth of FIH-1-depleted MDA-MB-231 cells[1].
Naphthofluorescein (compound 19) (100 mg/kg; i.p.; once daily for 5 consecutive days followed by 2 days off for 2 weeks) suppresses tumour growth in human cancer cells in an FIH-1-dependent manner[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male C57BL/6J mice (8 weeks)[1]
Dosage: 100 mg/kg
Administration: i.p.; once daily for 5 consecutive days followed by 2 days off for 2 weeks
Result: Neither showed weight loss or apparent histological abnormalities in the lung, liver, and kidney, or caused cause severe adverse effects for at least 2 weeks in mice.
Animal Model: Female C57BL/6J mice (E0771) and BALB/c nude mice (HT1080, MDA-MB-231, and AsPC-1) (6 weeks)[1]
Dosage: 100 mg/kg
Administration: i.p.; once daily for 5 consecutive days followed by 2 days off for 2 weeks
Result: Strikingly suppressed the tumour growth of subcutaneously injected E0771 cells and significantly attenuated tumour growth of MDA-MB-231 and AsPC-1 cells in immunodeficient mice. In turn, naphthofluorescein did not attenuate tumour growth of FIH-1-depleted MDA-MB-231 cells.
分子量

432.42

Formula

C28H16O5
CAS 号
性状

固体

颜色

Purple to purplish red

运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO 中的溶解度 : 125 mg/mL (289.07 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.3126 mL 11.5628 mL 23.1257 mL
5 mM 0.4625 mL 2.3126 mL 4.6251 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料
参考文献

Naphthofluorescein 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3126 mL 11.5628 mL 23.1257 mL 57.8142 mL
5 mM 0.4625 mL 2.3126 mL 4.6251 mL 11.5628 mL
10 mM 0.2313 mL 1.1563 mL 2.3126 mL 5.7814 mL
15 mM 0.1542 mL 0.7709 mL 1.5417 mL 3.8543 mL
20 mM 0.1156 mL 0.5781 mL 1.1563 mL 2.8907 mL
25 mM 0.0925 mL 0.4625 mL 0.9250 mL 2.3126 mL
30 mM 0.0771 mL 0.3854 mL 0.7709 mL 1.9271 mL
40 mM 0.0578 mL 0.2891 mL 0.5781 mL 1.4454 mL
50 mM 0.0463 mL 0.2313 mL 0.4625 mL 1.1563 mL
60 mM 0.0385 mL 0.1927 mL 0.3854 mL 0.9636 mL
80 mM 0.0289 mL 0.1445 mL 0.2891 mL 0.7227 mL
100 mM 0.0231 mL 0.1156 mL 0.2313 mL 0.5781 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Naphthofluorescein61419-02-1NaphthafluoresceinHIF/HIF Prolyl-HydroxylaseHypoxia-inducible factorsHIFsHIF-PHHIF-1Mint3cytotoxicityadverse effectfluorescent pH-sensitive probeantitumorInhibitorinhibitorinhibit

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称：

  

* 需求量：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Bulk Inquiry

Inquiry Information

产品名称：
Naphthofluorescein
目录号：
HY-124473
需求量：

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

   产品名称：

  

* Lot #：

* 客户姓名：

 

* Email：

   电话：

 

* 部门：

* 公司或机构名称：

 

   留言给我们：

Request for HNMR Report

We have received your request and will respond to you as soon as possible.

嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z