Rapamycin (GMP Like) (Synonyms: Sirolimus (GMP Like); AY-22989 (GMP Like); NSC 226080 (GMP Like))

目录号: HY-10219GL 含量: 98.8%: Data Sheet SDS COA 产品使用指南
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Rapamycin (Sirolimus) GMP Like 是 GMP Like 级别的 Rapamycin (HY-10219)。GMP Like 级别的小分子可用做细胞疗法中的辅助试剂。Rapamycin 是一种有效且特异性的 mTOR 抑制剂。

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Rapamycin (GMP Like) Chemical Structure

CAS No. : 53123-88-9

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生物活性
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参考文献

生物活性

Rapamycin (Sirolimus) GMP Like is Rapamycin (HY-10219) produced by using GMP like guidelines. GMP Like small molecules works appropriately as an auxiliary reagent for cell therapy manufacture. Rapamycin is a potent and specific mTOR inhibitor^[1]^[2]^[3].

细胞效力
(Cellular Effect)

Cell Line	Type	Value	Description	References
A549	IC₅₀	18.74 μM Compound: Rapamycin	Antiproliferative activity against human A549 cells by MTT assay Antiproliferative activity against human A549 cells by MTT assay	[PMID: 31546197]
A549	IC₅₀	30.72 μM Compound: Rapamycin	Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 33862514]
A549	IC₅₀	49.35 μM Compound: Rapamycin	Antitumor activity against human A549 cells assessed as cell growth inhibition incubated for 72 hrs by Cell Titer-Glo assay Antitumor activity against human A549 cells assessed as cell growth inhibition incubated for 72 hrs by Cell Titer-Glo assay	[PMID: 35688004]
C-33-A	IC₅₀	< 50 nM Compound: 25	Antiproliferative activity against human C-33-A cells assessed as inhibition of cell proliferation incubated for 5 to 7 days by MTS assay Antiproliferative activity against human C-33-A cells assessed as inhibition of cell proliferation incubated for 5 to 7 days by MTS assay	[PMID: 36669398]
DU-145	IC₅₀	4 μM Compound: Rapamycin	Antitumor activity against human DU-145 cells assessed as cell growth inhibition Antitumor activity against human DU-145 cells assessed as cell growth inhibition	[PMID: 35688004]
HCT-8	IC₅₀	1.25 μM Compound: Sirolimus	TP_TRANSPORTER: inhibition of Rhodamine 123 transport (basal to apical) (R123: 15 uM) in HCT-8 cells TP_TRANSPORTER: inhibition of Rhodamine 123 transport (basal to apical) (R123: 15 uM) in HCT-8 cells	[PMID: 12235265]
HEK-293T	EC₅₀	0.1 nM Compound: RAPA	Antiviral activity against HIV1 R5 assessed as inhibition of cell-cell fusion between R5-envelope expressing HEK293T cells and CD8 depleted human PBMCs pretreated for 6 days Antiviral activity against HIV1 R5 assessed as inhibition of cell-cell fusion between R5-envelope expressing HEK293T cells and CD8 depleted human PBMCs pretreated for 6 days	[PMID: 17485501]
HEK-293T	EC₅₀	1.3 nM Compound: RAPA	Antiviral activity against HIV1 X4 assessed as inhibition of cell-cell fusion between X4-envelope expressing HEK293T cells and CD8 depleted human PBMCs pretreated for 6 days Antiviral activity against HIV1 X4 assessed as inhibition of cell-cell fusion between X4-envelope expressing HEK293T cells and CD8 depleted human PBMCs pretreated for 6 days	[PMID: 17485501]
HEK293	EC₅₀	0.05 μM Compound: Rapamycin	Inhibition of TPA-induced degradation of Pdcd4 (amino acid 39-91) expressed in human HEK293 cells assessed as minimum compound concentration required for 50% recovery of Pdcd4-luciferase signal incubated for 8 hrs by luciferase reporter gene assay Inhibition of TPA-induced degradation of Pdcd4 (amino acid 39-91) expressed in human HEK293 cells assessed as minimum compound concentration required for 50% recovery of Pdcd4-luciferase signal incubated for 8 hrs by luciferase reporter gene assay	[PMID: 21539301]
HEK293	IC₅₀	0.1 nM Compound: Rapamycin	Inhibition of mTOR in HEK293 cells Inhibition of mTOR in HEK293 cells	[PMID: 32324396]
HEK293	IC₅₀	0.1 nM Compound: rapamycin	Inhibition of mTOR kinase expressed in human HEK293 cells by Western blot analysis Inhibition of mTOR kinase expressed in human HEK293 cells by Western blot analysis	[PMID: 17350953]
HEK293	IC₅₀	1.1 μM Compound: Rapamycin	TP_TRANSPORTER: inhibition of Phalloidin uptake (Phalloidin: 1 uM) in OATP-C-expressing HEK293 cells TP_TRANSPORTER: inhibition of Phalloidin uptake (Phalloidin: 1 uM) in OATP-C-expressing HEK293 cells	[PMID: 14530907]
HT-3	IC₅₀	< 50 nM Compound: 25	Antiproliferative activity against human HT-3 cells assessed as inhibition of cell proliferation incubated for 5 to 7 days by MTS assay Antiproliferative activity against human HT-3 cells assessed as inhibition of cell proliferation incubated for 5 to 7 days by MTS assay	[PMID: 36669398]
HeLa	IC₅₀	0.77 μM Compound: Rapamycin	Cytotoxicity against human HeLa cells Cytotoxicity against human HeLa cells	[PMID: 30015070]
HeLa	IC₅₀	> 10000 nM Compound: Rapamycin	Growth inhibition of human HeLa cells after 72 hrs by CCK8 assay Growth inhibition of human HeLa cells after 72 hrs by CCK8 assay	[PMID: 29308895]
HepG2	CC₅₀	67.2 μM Compound: 78	Cytotoxicity against human HepG2 assessed as reduction in cell viability after 24 hrs by CellTiter-Glo luminescent assay Cytotoxicity against human HepG2 assessed as reduction in cell viability after 24 hrs by CellTiter-Glo luminescent assay	[PMID: 28051303]
HepG2	EC₅₀	10 μM Compound: Sirolimus	Activation of human PXR expressed in human HepG2 (DPX-2) cells assessed as induction of CYP3A4 after 24 hrs by luminescent analysis Activation of human PXR expressed in human HepG2 (DPX-2) cells assessed as induction of CYP3A4 after 24 hrs by luminescent analysis	[PMID: 20966043]
K562	IC₅₀	1 mM Compound: rapamycin	Antiproliferative activity against human K562 cells Antiproliferative activity against human K562 cells	[PMID: 18571290]
MCF7	EC₅₀	< 1 nM Compound: Rapamycin	Cytotoxicity against human MCF7 cells after 5 days by Presto blue reagent-based fluorescence analysis Cytotoxicity against human MCF7 cells after 5 days by Presto blue reagent-based fluorescence analysis	10.1039/C5MD00493D
MCF7	IC₅₀	18.74 μM Compound: Rapamycin	Antiproliferative activity against human MCF7 cells by MTT assay Antiproliferative activity against human MCF7 cells by MTT assay	[PMID: 31546197]
MCF7	IC₅₀	4980 nM Compound: Rapamycin	Growth inhibition of human MCF7 cells after 72 hrs by CCK8 assay Growth inhibition of human MCF7 cells after 72 hrs by CCK8 assay	[PMID: 29308895]
MDA-MB-231	GI₅₀	> 100 nM Compound: Rapamycin	Antiproliferative activity against human MDA-MB-231 cells incubated for 48 hrs by sulforhodamine B assay Antiproliferative activity against human MDA-MB-231 cells incubated for 48 hrs by sulforhodamine B assay	[PMID: 32510949]
MDA-MB-231	IC₅₀	0.9 μM Compound: Rapamycin	Antiproliferative activity against human MDA-MB-231 cells assessed as cell viability after 24 hrs Antiproliferative activity against human MDA-MB-231 cells assessed as cell viability after 24 hrs	[PMID: 33139111]
MDA-MB-231	IC₅₀	35.79 μM Compound: Rapamycin	Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 33862514]
MDA-MB-231	IC₅₀	> 10000 nM Compound: Rapamycin	Growth inhibition of human MDA-MB-231 cells after 72 hrs by CCK8 assay Growth inhibition of human MDA-MB-231 cells after 72 hrs by CCK8 assay	[PMID: 29308895]
MDA-MB-453	GI₅₀	0.05 nM Compound: Rapamycin	Antiproliferative activity against human MDA-MB-453 cells incubated for 48 hrs by sulforhodamine B assay Antiproliferative activity against human MDA-MB-453 cells incubated for 48 hrs by sulforhodamine B assay	[PMID: 32510949]
MDA-MB-468	IC₅₀	37.2 μM Compound: Rapamycin	Antiproliferative activity against human MDA-MB-468 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Antiproliferative activity against human MDA-MB-468 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 33862514]
ME-180	IC₅₀	< 50 nM Compound: 25	Antiproliferative activity against human ME-180 cells assessed as inhibition of cell proliferation incubated for 5 to 7 days by MTS assay Antiproliferative activity against human ME-180 cells assessed as inhibition of cell proliferation incubated for 5 to 7 days by MTS assay	[PMID: 36669398]
MEF	IC₅₀	0.05 nM Compound: Sirolimus	Inhibition of mTOR in mouse TSC1-/- MEF cells assessed as inhibition of S6 Ser240/244 phosphorylation incubated for 2 hrs by fluorescence based assay Inhibition of mTOR in mouse TSC1-/- MEF cells assessed as inhibition of S6 Ser240/244 phosphorylation incubated for 2 hrs by fluorescence based assay	[PMID: 31223435]
MV4-11	IC₅₀	> 500 nM Compound: RAPA	Antiproliferative activity against human MV4-11 cells after 72 hrs by MTT assay Antiproliferative activity against human MV4-11 cells after 72 hrs by MTT assay	[PMID: 30629434]
NCI-H460	IC₅₀	> 10000 nM Compound: Rapamycin	Growth inhibition of human H460 cells after 72 hrs by CCK8 assay Growth inhibition of human H460 cells after 72 hrs by CCK8 assay	[PMID: 29308895]
OCI-AML-3	IC₅₀	> 500 nM Compound: RAPA	Antiproliferative activity against human OCI-AML3 cells after 72 hrs by MTT assay Antiproliferative activity against human OCI-AML3 cells after 72 hrs by MTT assay	[PMID: 30629434]
OCI-AML2	IC₅₀	> 500 nM Compound: RAPA	Antiproliferative activity against human OCI-AML2 cells after 72 hrs by MTT assay Antiproliferative activity against human OCI-AML2 cells after 72 hrs by MTT assay	[PMID: 30629434]
OVCAR-5	IC₅₀	> 10000 nM Compound: Rapamycin	Growth inhibition of human OVCAR5 cells after 72 hrs by CCK8 assay Growth inhibition of human OVCAR5 cells after 72 hrs by CCK8 assay	[PMID: 29308895]
OVCAR-8	IC₅₀	< 50 nM Compound: 25	Antiproliferative activity against human OVCAR-8 cells assessed as inhibition of cell proliferation incubated for 5 to 7 days by MTS assay Antiproliferative activity against human OVCAR-8 cells assessed as inhibition of cell proliferation incubated for 5 to 7 days by MTS assay	[PMID: 36669398]
PC-3	EC₅₀	43.1 μM Compound: Rapamycin	Cytotoxicity against human PC3 cells by MTT assay Cytotoxicity against human PC3 cells by MTT assay	[PMID: 28774426]
PC-3	IC₅₀	1.3 μM Compound: Rapamycin	Antitumor activity against human PC-3 cells assessed as cell growth inhibition Antitumor activity against human PC-3 cells assessed as cell growth inhibition	[PMID: 35688004]
PC-3	IC₅₀	< 0.01 μM Compound: Rapamycin	Antiproliferative activity against human PC3 cells Antiproliferative activity against human PC3 cells	[PMID: 21978683]
PC-3	IC₅₀	< 0.01 μM Compound: Rapamycin	Inhibition of mTOR-mediated S6 phosphorylation in human PC3 cells Inhibition of mTOR-mediated S6 phosphorylation in human PC3 cells	[PMID: 21978683]
PC-3	IC₅₀	> 10 μM Compound: Rapamycin	Inhibition of mTOR-mediated Akt phosphorylation in human PC3 cells Inhibition of mTOR-mediated Akt phosphorylation in human PC3 cells	[PMID: 21978683]
SF-268	IC₅₀	2.7 μM Compound: Rapamycin	Antitumor activity against human SF-268 cells assessed as cell growth inhibition Antitumor activity against human SF-268 cells assessed as cell growth inhibition	[PMID: 35688004]
SK-MEL-2	IC₅₀	1 μM Compound: Rapamycin	Synergistic cytotoxicity against human SK-MEL-2 cells expressing NRAS mutant assessed as cell growth inhibition measured for 48 hrs in presence of LY3214996 by MTT assay Synergistic cytotoxicity against human SK-MEL-2 cells expressing NRAS mutant assessed as cell growth inhibition measured for 48 hrs in presence of LY3214996 by MTT assay	[PMID: 36961300]
SK-MEL-2	IC₅₀	1 μM Compound: Rapamycin	Synergistic cytotoxicity against human SK-MEL-2 cells expressing NRAS mutant assessed as cell growth inhibition measured for 48 hrs in presence of MEK-162 by MTT assay Synergistic cytotoxicity against human SK-MEL-2 cells expressing NRAS mutant assessed as cell growth inhibition measured for 48 hrs in presence of MEK-162 by MTT assay	[PMID: 36961300]
SK-OV-3	IC₅₀	< 50 nM Compound: 25	Antiproliferative activity against human SK-OV-3 cells assessed as inhibition of cell proliferation incubated for 5 to 7 days by MTS assay Antiproliferative activity against human SK-OV-3 cells assessed as inhibition of cell proliferation incubated for 5 to 7 days by MTS assay	[PMID: 36669398]
SK-OV-3	IC₅₀	> 10000 nM Compound: Rapamycin	Growth inhibition of human SKOV3 cells after 72 hrs by CCK8 assay Growth inhibition of human SKOV3 cells after 72 hrs by CCK8 assay	[PMID: 29308895]
SUM185PE	GI₅₀	0.04 nM Compound: Rapamycin	Antiproliferative activity against human SUM185PE cells incubated for 48 hrs by sulforhodamine B assay Antiproliferative activity against human SUM185PE cells incubated for 48 hrs by sulforhodamine B assay	[PMID: 32510949]
SiHa	IC₅₀	1756 nM Compound: Rapamycin	Growth inhibition of human SiHa cells after 72 hrs by CCK8 assay Growth inhibition of human SiHa cells after 72 hrs by CCK8 assay	[PMID: 29308895]
T-cell	IC₅₀	1.6 nM Compound: 1	T-cell antiproliferative activity in human mixed lymphocyte reaction T-cell antiproliferative activity in human mixed lymphocyte reaction	[PMID: 16185865]
T-cell	IC₅₀	2600 nM Compound: 1	Antiproliferative activity in Lewis rat lymph node cells in rat mixed lymphocyte reaction Antiproliferative activity in Lewis rat lymph node cells in rat mixed lymphocyte reaction	[PMID: 16185865]
T-cell	IC₅₀	9.8 nM Compound: 1	Tested for inhibition of T cell proliferation in the co-mitogen stimulated proliferation assay Tested for inhibition of T cell proliferation in the co-mitogen stimulated proliferation assay	10.1016/0960-894X(95)00223-G
T47D	IC₅₀	1576 nM Compound: Rapamycin	Growth inhibition of human T47D cells after 72 hrs by CCK8 assay Growth inhibition of human T47D cells after 72 hrs by CCK8 assay	[PMID: 29308895]
U-87MG ATCC	IC₅₀	10 μM Compound: Rapamycin	Antitumor activity against human U-87 MG cells assessed as cell growth inhibition Antitumor activity against human U-87 MG cells assessed as cell growth inhibition	[PMID: 35688004]

体外研究
(In Vitro)

Rapamycin GMP Like（1 nM；2-3 周）可诱导人胚胎干细胞 (hESC) 体外分化为矿化成骨细胞^[1]。
Rapamycin GMP Like（1 nM；2-3 周）可增强人胚状体 (hEB) 的成骨细胞分化^[1]。
Rapamycin GMP Like（20 nM；4 小时）血清依赖性促进血管平滑肌细胞 (VSMC) 分化^[2]。
Rapamycin GMP Like 诱导大鼠成骨样骨肉瘤细胞中的成骨细胞分化^[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

914.17

Formula

C₅₁H₇₉NO₁₃

CAS 号

53123-88-9

性状

固体

颜色

White to off-white

中文名称

雷帕霉素 (GMP Like)；西罗莫司 (GMP Like)

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

纯度 & 产品资料

纯度: 98.8%

选择批次：

Data Sheet (538 KB) SDS (252 KB)

COA (272 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Lee KW, et al. Rapamycin promotes the osteoblastic differentiation of human embryonic stem cells by blocking the mTOR pathway and stimulating the BMP/Smad pathway. Stem Cells Dev. 2010 Apr;19(4):557-68. [Content Brief] [Content Brief]

[2]. Martin KA, et al. Rapamycin promotes vascular smooth muscle cell differentiation through insulin receptor substrate-1/phosphatidylinositol 3-kinase/Akt2 feedback signaling. J Biol Chem. 2007 Dec 7;282(49):36112-20. [Content Brief] [Content Brief]

[3]. Ogawa T, et al. Osteoblastic differentiation is enhanced by rapamycin in rat osteoblast-like osteosarcoma (ROS 17/2.8) cells. Biochem Biophys Res Commun. 1998 Aug 10;249(1):226-30. [Content Brief] [Content Brief]

Rapamycin (GMP Like) 相关分类

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The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Rapamycin (GMP Like) (Synonyms: Sirolimus (GMP Like); AY-22989 (GMP Like); NSC 226080 (GMP Like))

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Rapamycin (GMP Like) 相关分类

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Rapamycin (GMP Like) (Synonyms: Sirolimus (GMP Like); AY-22989 (GMP Like); NSC 226080 (GMP Like))

Customer Review

Other Forms of Rapamycin (GMP Like):

Rapamycin (GMP Like) 相关抗体

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Rapamycin (GMP Like) 相关分类

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