ABCD2 蛋白, Human (Cell-Free, His)

ABCD2 protein is an ATP-dependent transporter in the ABC family that promotes the transport of very long chain fatty acids (VLCFA)-CoA into peroxisomes and exhibits fatty acyl-CoA thioesterase (ACOT) and ATPase activities. Its substrate specificity overlaps with ABCD1, including a clear preference for shorter VLCFA (C22:0) and polyunsaturated fatty acids (PUFA) such as C22:6-CoA and C24:6-CoA. ABCD2 Protein, Human (Cell-Free, His) is the recombinant human-derived ABCD2 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of ABCD2 Protein, Human (Cell-Free, His) is 740 a.a., with molecular weight of 89.3 kDa.

作为属于 ATP 结合盒 (ABC) 家族的 ATP 依赖性转运蛋白，ABCD2 蛋白是将超长链脂肪酸 (VLCFA)-CoA 从细胞质转运到过氧化物酶体腔的过程中不可或缺的一部分。最近的研究表明，与 ABCD1 类似，ABCD2 也表现出脂肪酰辅酶 A 硫酯酶 (ACOT) 和 ATP 酶活性。根据该模型，VLCFA-CoA 在 ABCD2 的 ACOT 活性介导下水解后，以 ATP 依赖性方式转运至过氧化物酶体中（可能）。ABCD2 的底物特异性与 ABCD1 重叠，特别是对饱和脂肪酸 (FA) 和单不饱和 FA (MUFA)，但它表现出对较短的 VLCFA (C22:0) 和多不饱和脂肪酸 (PUFA) 如 C22:6 的明显偏好-CoA 和 C24:6-CoA 体外。这表明 ABCD2 在调节极长链脂肪酸 (VLCFA) 和能量代谢中具有潜在作用，特别是在脂肪酸通过 β-氧化的降解和生物合成中。

Human

E. coli Cell-free

N-10*His

Q9UBJ2 (M1-S740)

225

MTHMLNAAADRVKWTRSSAAKRAACLVAAAYALKTLYPIIGKRLKQSGHGKKKAAAYPAAENTEILHCTETICEKPSPGVNADFFKQLLELRKILFPKLVTTETGWLCLHSVALISRTFLSIYVAGLDGKIVKSIVEKKPRTFIIKLIKWLMIAIPATFVNSAIRYLECKLALAFRTRLVDHAYETYFTNQTYYKVINMDGRLANPDQSLTEDIMMFSQSVAHLYSNLTKPILDVMLTSYTLIQTATSRGASPIGPTLLAGLVVYATAKVLKACSPKFGKLVAEEAHRKGYLRYVHSRIIANVEEIAFYRGHKVEMKQLQKSYKALADQMNLILSKRLWYIMIEQFLMKYVWSSSGLIMVAIPIITATGFADGEDGQKQVMVSERTEAFTTARNLLASGADAIERIMSSYKEVTELAGYTARVYNMFWVFDEVKRGIYKRTAVIQESESHSKNGAKVELPLSDTLAIKGKVIDVDHGIICENVPIITPAGEVVASRLNFKVEEGMHLLITGPNGCGKSSLFRILSGLWPVYEGVLYKPPPQHMFYIPQRPYMSLGSLRDQVIYPDSVDDMHDKGYTDQDLERILHNVHLYHIVQREGGWDAVMDWKDVLSGGEKQRMGMARMFYHKPKYALLDECTSAVSIDVEGKIFQAAKGAGISLLSITHRPSLWKYHTHLLQFDGEGGWRFEQLDTAIRLTLSEEKQKLESQLAGIPKMQQRLNELCKILGEDSVLKTIKNEDETS

89.3 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.