Animal-Free CHI3L1 蛋白, Human (His)

Chitinase-3-like protein 1 (CHI3L1) is a carbohydrate-binding lectin with a specific affinity for chitin, although it lacks chitinase activity. Its main role extends beyond chitin degradation, as it is implicated in tissue remodeling and cellular responses to environmental changes. CHI3L1 plays a crucial role in T-helper cell type 2 (Th2) inflammatory responses and IL-13-induced inflammation, influencing allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation, and the differentiation of M2 macrophages. Additionally, it facilitates the invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. CHI3L1 is involved in the activation of the AKT1 signaling pathway, leading to IL8 production in colonic epithelial cells. Furthermore, it regulates antibacterial responses in the lungs, contributing to macrophage bacterial killing, controlling bacterial dissemination, and enhancing host tolerance. In lung tissues, CHI3L1 also plays a role in regulating hyperoxia-induced injury, inflammation, and epithelial apoptosis. Structurally, CHI3L1 functions as a monomer. Animal-Free CHI3L1 Protein, Human (His) is the recombinant human-derived animal-FreeCHI3L1 protein, expressed by E. coli , with C-His labeled tag. The total length of Animal-Free CHI3L1 Protein, Human (His) is 362 a.a., with molecular weight of ~41.43 kDa.

几丁质酶 3 样蛋白 1 (CHI3L1) 是一种碳水化合物结合凝集素，对几丁质具有特异性亲和力，尽管它缺乏几丁质酶活性。它的主要作用超出了几丁质降解，因为它涉及组织重塑和细胞对环境变化的反应。 CHI3L1 在 2 型辅助性 T 细胞 (Th2) 炎症反应和 IL-13 诱导的炎症反应中发挥着至关重要的作用，影响过敏原致敏、炎症细胞凋亡、树突状细胞积累和 M2 巨噬细胞的分化。此外，它还促进致病性肠道细菌侵入结肠粘膜和淋巴器官。 CHI3L1 参与 AKT1 信号通路的激活，导致结肠上皮细胞产生 IL8。此外，它还调节肺部的抗菌反应，有助于杀死巨噬细胞细菌、控制细菌传播并增强宿主耐受性。在肺组织中，CHI3L1 还在调节高氧诱导的损伤、炎症和上皮细胞凋亡中发挥作用。在结构上，CHI3L1 作为单体发挥作用。

Human

E. coli

C-His

P36222 (Y22-T383)

1116  [NCBI]

MYKLVCYYTSWSQYREGDGSCFPDALDRFLCTHIIYSFANISNDHIDTWEWNDVTLYGMLNTLKNRNPNLKTLLSVGGWNFGSQRFSKIASNTQSRRTFIKSVPPFLRTHGFDGLDLAWLYPGRRDKQHFTTLIKEMKAEFIKEAQPGKKQLLLSAALSAGKVTIDSSYDIAKISQHLDFISIMTYDFHGAWRGTTGHHSPLFRGQEDASPDRFSNTDYAVGYMLRLGAPASKLVMGIPTFGRSFTLASSETGVGAPISGPGIPGRFTKEAGTLAYYEICDFLRGATVHRILGQQVPYATKGNQWVGYDDQESVKSKVQYLKDRQLAGAMVWALDLDDFQGSFCGQDLRFPLTNAIKDALAAT

Approximately 41.43 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a solution containing 1X PBS, pH 7.4.

<0.1 EU per 1 μg of the protein by the LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.