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  4. AQP2 蛋白, Mouse (Cell-Free, His)

AQP2 蛋白, Mouse (Cell-Free, His)

目录号: HY-P702213
产品使用指南

AQP2 是一种同源四聚体蛋白，在肾水稳态中发挥关键作用。它充当水特异性通道，促进水穿过肾集合管质膜的运动。AQP2 蛋白, Mouse (Cell-Free, His) 是重组的 AQP2 蛋白，由 E. coli Cell-free 表达，带有 N-10*His 标签。AQP2 蛋白, Mouse (Cell-Free, His) 全长 271 个氨基酸，分子量为 30.5 kDa。

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100 μg   询价  

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描述

AQP2 is a homotetrameric protein that plays a key role in renal water homeostasis. It acts as a water-specific channel, facilitating the movement of water across the plasma membrane of the renal collecting duct. AQP2 Protein, Mouse (Cell-Free, His) is the recombinant mouse-derived AQP2 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of AQP2 Protein, Mouse (Cell-Free, His) is 271 a.a., with molecular weight of 30.5 kDa.
研究背景

AQP2 是一种同源四聚体蛋白，在肾水稳态中发挥关键作用。它充当水特异性通道，促进水穿过肾集合管质膜的运动。通过赋予水高渗透性，AQP2 使水能够响应渗透梯度而流动，从而有助于调节肾脏中的水平衡。它在维持适当水位方面的关键功能强调了它在肾脏生理学中的重要性，并强调了它在整体体液调节中的重要性。
种属

Mouse
表达系统

E. coli Cell-free
标签

N-10*His
蛋白编号

P56402 (M1-A271)
基因 ID

11827
蛋白结构
N-term
10*His
AQP2 (M1-A271)
Accession # P56402
C-term
同用名
Aquaporin-2; ADH water channel; Aquaporin-CD; AQP-CD; Collecting duct water channel protein; WCH-CD; Water channel protein for renal collecting duct
氨基酸序列

MWELRSIAFSRAVLAEFLATLLFVFFGLGSALQWASSPPSVLQIAVAFGLGIGTLVQALGHVSGAHINPAVTVACLVGCHVSFLRAAFYVAAQLLGAVAGAAILHEITPVEIRGDLAVNALHNNATAGQAVTVELFLTMQLVLCIFASTDERRSDNLGSPALSIGFSVTLGHLLGIYFTGCSMNPARSLAPAVVTGKFDDHWVFWIGPLVGAVIGSLLYNYLLFPSTKSLQERLAVLKGLEPDTDWEEREVRRRQSVELHSPQSLPRGSKA

分子量

30.5 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

AQP2 Protein, Mouse (Cell-Free, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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