1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. ASTL/SAS1B 蛋白, Mouse (His)

ASTL/SAS1B 蛋白, Mouse (His)

目录号: HY-P77566
产品使用指南

ASTL/SAS1B 蛋白是一种卵母细胞特异性受体,在精卵粘附中发挥着关键作用,这对受精至关重要。它抑制多精,防止多个精子使一个卵子受精。ASTL/SAS1B 可能充当受精后蛋白酶,在透明带中裂解 ZP2。这种分裂发生在受精和皮质颗粒胞吐作用之后,使透明带不支持额外的精子结合,从而调节受精过程。ASTL/SAS1B 蛋白, Mouse (His) 是重组的 ASTL/SAS1B 蛋白,由 E. coli 表达,带有 N-His 标签。ASTL/SAS1B 蛋白, Mouse (His) 全长 412 个氨基酸,分子量约为 ~46.2 kDa。

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描述

ASTL/SAS1B Protein, an oocyte-specific receptor, plays a pivotal role in sperm-egg adhesion, crucial for fertilization. It inhibits polyspermy, preventing multiple sperm from fertilizing one egg. ASTL/SAS1B likely acts as a post-fertilization protease, cleaving ZP2 in the zona pellucida. This cleavage, occurring after fertilization and cortical granule exocytosis, renders the zona pellucida unsupportive of additional sperm binding, regulating fertilization processes. ASTL/SAS1B Protein, Mouse (His) is the recombinant mouse-derived ASTL/SAS1B protein, expressed by E. coli, with N-His labeled tag. The total length of ASTL/SAS1B Protein, Mouse (His) is 412 a.a., with molecular weight of ~46.2 kDa.

研究背景

ASTL/SAS1B 蛋白作为卵母细胞特异性卵膜受体,对精子和卵子的粘附至关重要,在受精过程中发挥着关键作用。这种蛋白质在抑制多精受精方面具有重要意义,表明它参与阻止多个精子使单个卵子受精。ASTL/SAS1B 可能充当蛋白酶,负责 ZP2(透明带的一个组成部分)的受精后裂解。ZP2 的裂解发生在受精和皮质颗粒胞吐作用后的透明带表面。这种酶活性使得透明带无法支持进一步的精子结合,从而有助于受精过程的调节。

种属

Mouse

表达系统

E. coli

标签

N-His

蛋白编号

Q6HA09 (A24-D435)

基因 ID

215095  [NCBI]

蛋白结构
N-term
His
ASTL (A24-D435)
Accession # Q6HA09
C-term
同用名
Oocyte astacin; Ovastacin; Sas1b; Astl; ASTL
分子量

Approximately 46.2 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

ASTL/SAS1B Protein, Mouse (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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