B7-H2/ICOSLG 蛋白, Human (Biotinylated, HEK293, His-Avi)

B7-H2/ICOSLG Protein, a ligand for T-cell receptor ICOS, critically costimulates T-cell proliferation and cytokine secretion. It induces B-cell proliferation and supports plasma cell differentiation, playing a key role in local tissue responses to inflammation. B7-H2/ICOSLG also modulates the secondary immune response by co-stimulating memory T-cell function. In molecular interactions, it has been observed to interact with CTLA4 in vitro. B7-H2/ICOSLG Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived B7-H2/ICOSLG protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of B7-H2/ICOSLG Protein, Human (Biotinylated, HEK293, His-Avi) is 240 a.a., with molecular weight of 55-70 kDa.

B7-H2/ICOSLG 蛋白作为 T 细胞特异性细胞表面受体 ICOS 的配体，充当 T 细胞增殖和细胞因子分泌的关键共刺激信号。此外，它还能诱导 B 细胞增殖并促进其分化为浆细胞。这种蛋白质有望在介导局部组织对炎症反应的反应以及通过共同刺激记忆 T 细胞功能来调节二次免疫反应方面发挥重要作用。在分子相互作用中，B7-H2/ICOSLG 已被证明可以在体外与 CTLA4 相互作用。

1. Immobilized Biotinylated Human B7-H2 His at 0.5 μg/mL (100 μL/Well) on the plate. Dose response curve for Human ICOS hFc with the EC₅₀ of 21.1 ng/mL determined by ELISA.
2. Immobilized Human ICOS (C136S,C137S) , His Tag at 1 μg/mL (100 μl/well) on the plate. Dose response curve for Biotinylated Human B7-H2, His Tag with the EC₅₀ of 104.0 ng/mL determined by ELISA.

Human

HEK293

C-Avi;C-His

O75144 (D19-S258)

23308  [NCBI]

55-70 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.