Biliverdin Reductase A/BLVRA 蛋白, Human (N-His)

Biliverdin Reductase A/BLVRA catalyzes the reduction of biliverdin IX alpha to bilirubin, utilizing either NADH or NADPH as a cofactor. The pH-dependent preference involves NADH at acidic pH (6-6.9) and NADPH at alkaline pH (8.5-8.7), with NADPH being the predominant reactant in biological systems. This enzymatic activity plays a crucial role in heme catabolism, influencing physiological processes and cellular redox balance. Biliverdin Reductase A/BLVRA Protein, Human (N-His) is the recombinant human-derived Biliverdin Reductase A/BLVRA protein, expressed by E. coli , with N-6*His labeled tag. The total length of Biliverdin Reductase A/BLVRA Protein, Human (N-His) is 289 a.a., with molecular weight of ~40 kDa.

胆绿素还原酶 A/BLVRA 蛋白充当开放四吡咯胆绿素 IX α 的 γ-亚甲基桥还原为胆红素的催化剂，同时伴有 NADH 或 NADPH 辅因子的氧化。NADH 和 NADPH 作为辅因子的选择取决于 pH 值，NADH 在酸性 pH 范围 (6-6.9) 中使用，NADPH 在碱性范围 (8.5-8.7) 中使用。然而，在生物系统中，NADPH 被认为是可能且更普遍的反应物。这种酶活性凸显了 BLVRA 在胆绿素转化为胆红素中的关键作用，这是血红素分解代谢的关键步骤，对各种生理过程和细胞氧化还原平衡都有影响。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

P53004 (E6-S294)

644  [NCBI]

ERKFGVVVVGVGRAGSVRMRDLRNPHPSSAFLNLIGFVSRRELGSIDGVQQISLEDALSSQEVEVAYICSESSSHEDYIRQFLNAGKHVLVEYPMTLSLAAAQELWELAEQKGKVLHEEHVELLMEEFAFLKKEVVGKDLLKGSLLFTAGPLEEERFGFPAFSGISRLTWLVSLFGELSLVSATLEERKEDQYMKMTVCLETEKKSPLSWIEEKGPGLKRNRYLSFHFKSGSLENVPNVGVNKNIFLKDQNIFVQKLLGQFSEKELAAEKKRILHCLGLAEEIQKYCCS

Approximately 40 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For lower concentration,please reconstitute in 50 mM Tris-HCL,300 mM NaCl, pH 7.4 buffer.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.