BMP-5 蛋白, Human (HEK293, hFc)

Bone morphogenetic protein 5 (BMP-5) is a pleiotropic ligand protein belonging to the TGFβ family, which is mainly expressed in the lung and liver. After BMP-5 silencing, the activity of p38/ERK signaling pathway can be down-regulated to inhibit chondrocyte senescence and apoptosis and knee arthritis (OA)^[1]. Bmp-5 initiates typical BMP signaling cascades by binding to type I receptor BMPR1A and type II receptor BMPR2^[2], or triggers signals through atypical pathways such as MAPK p38 signaling cascades to promote chondrogenic differentiation^[3]. The total length of human BMP-5 protein is 454 amino acids (M1-H454), with 4 glycosylation domains. BMP-5 Protein, Human (HEK293, hFc) has a total length of 131 amino acids (Q324-H454), is expressed in HEK293 cells with a N-terminal hFc-tag.

骨形态发生蛋白5（BMP-5）是一种多效性配体蛋白，属于TGFβ家族，主要在肺和肝脏中表达。BMP/TGFβ 信号传导参与血管和瓣膜稳态，这是胚胎发育的关键过程^[4]。BMP/TGFβ 信号传导可被包括 SMAD6 和 SMAD7 在内的抑制性 SMAD 终止，这些 SMAD 被 BMP 信号传导激活和诱导，并通过多种机制关闭 BMP 信号传导^[5]。
BMP-5沉默可通过下调 p38/ERK 信号通路的活性来抑制软骨细胞衰老和凋亡以及骨关节炎 (OA) 进展^[1]。
BMP-5 通过关联与 I 型受体 BMPR1A 和 II 型受体 BMPR2^[2] 结合。
BMP-5 还可通过非经典途径（例如 MAPK p38 信号级联）触发信号，促进软骨分化^[3]。
BMP-5在神经嵴祖细胞中的特异性表达足以通过MEK-ERK-ID3信号级联诱导细胞增殖，而破坏该信号级联对细胞存活没有影响^[6]。

BMP-5 (3 ng/mL; 1, 3, 5, 7, and 17 d) induces SCG neurons dendritic growth^[2].
BMP-5 (1 ng/mL; , 3, and 6 min) induces phosphorylation of Smad-1 in cultured sympathetic neurons^[2].
BMP-5 (.1-1 ng/mL; 6 d) is less potent than BMP-7 in dendritic growth in cultured sympathetic neurons, but equally efficacious in promoting dendritic growth in cultured sympathetic neurons^[2].

Human

HEK293

N-hFc

NP_066551.1 (Q324-H454)

653  [NCBI]

50-55 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Shao Y, et al. BMP5 silencing inhibits chondrocyte senescence and apoptosis as well as osteoarthritis progression in mice. Aging (Albany NY). 2021 Mar 19;13(7):9646-9664.  [Content Brief]

  [2]. Beck HN, et al. Bone morphogenetic protein-5 (BMP-5) promotes dendritic growth in cultured sympathetic neurons. BMC Neurosci. 2001;2:12.  [Content Brief]

  [3]. Snelling SJ, et al. BMP5 activates multiple signaling pathways and promotes chondrogenic differentiation in the ATDC5 growth plate model. Growth Factors. 2010 Aug;28(4):268-79.  [Content Brief]

  [4]. Yang P, et al. The role of bone morphogenetic protein signaling in vascular calcification. Bone. 2020 Dec;141:115542.  [Content Brief]

  [5]. Miyazawa K, et al. Regulation of TGF-β Family Signaling by Inhibitory Smads. Cold Spring Harb Perspect Biol. 2017 Mar 1;9(3):a022095.  [Content Brief]

  [6]. Shih HY, et al. Bmp5 Regulates Neural Crest Cell Survival and Proliferation via Two Different Signaling Pathways. Stem Cells. 2017 Apr;35(4):1003-1014.  [Content Brief]