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  3. CACNG1 蛋白, Mouse (Cell-Free, His)

CACNG1 蛋白, Mouse (Cell-Free, His)

目录号: HY-P702229
产品使用指南

CACNG1 蛋白是电压门控钙通道的调节亚基,有助于骨骼肌中的 L 型钙电流并调节通道失活动力学。CACNG1 与 CACNA1S、CACNB1 或 CACNB2 以及 CACNA2D1 合作,协调平衡的 1:1:1:1 比例通道组装,影响骨骼肌中电压门控钙通道的功能。CACNG1 蛋白, Mouse (Cell-Free, His) 是重组的 CACNG1 蛋白,由 E. coli Cell-free 表达,带有 N-10*His 标签。CACNG1 蛋白, Mouse (Cell-Free, His) 全长 223 个氨基酸,分子量为 26.6 kDa。

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描述

CACNG1 Protein, a regulatory subunit of the voltage-gated calcium channel, contributes to L-type calcium currents in skeletal muscle and modulates channel inactivation kinetics. Collaborating with CACNA1S, CACNB1 or CACNB2, and CACNA2D1, CACNG1 orchestrates a balanced 1:1:1:1 ratio channel assembly, influencing the voltage-gated calcium channel's functionality in skeletal muscle. CACNG1 Protein, Mouse (Cell-Free, His) is the recombinant mouse-derived CACNG1 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of CACNG1 Protein, Mouse (Cell-Free, His) is 223 a.a., with molecular weight of 26.6 kDa.

研究背景

CACNG1 蛋白作为电压门控钙通道的调节亚基,有助于骨骼肌中 L 型钙电流的产生。其关键作用延伸至通道失活动力学的调节。在钙通道复合体中,CACNG1 与成孔 α 亚基 (CACNA1S) 和其他辅助亚基(即 CACNB1 或 CACNB2 和 CACNA2D1)协作。这种复杂的通道组件由 α、β、γ 和 δ 亚基以 1:1:1:1 的平衡比例组成,其中包括 CACNB1 或 CACNB2。这些成分精心策划的相互作用凸显了 CACNG1 参与塑造骨骼肌电压门控钙通道的功能和特性。

种属

Mouse

表达系统

E. coli Cell-free

标签

N-10*His

蛋白编号

O70578 (M1-H223)

基因 ID

12299

蛋白结构
N-term
10*His
CACNG1 (M1-H223)
Accession # O70578
C-term
同用名
Voltage-dependent calcium channel gamma-1 subunit; Dihydropyridine-sensitive L-type, skeletal muscle calcium channel subunit gamma
氨基酸序列

MSQTKTAKVRVTLFFILVGGVLAMVAVVTDHWAVLSPHLEHHNETCEAAHFGLWRICTARVAVHNKDKSCEHVTPSGEKNCSYFRHFNPGESSEIFEFTTQKEYSISAAAIAIFSLGFIIVGSICAFLSFGNKRDYLLRPASMFYAFAGLCLIVSVEVMRQSVKRMIDSEDTVWIEHYYSWSFACACAAFILLFLGGLFLLLFSLPRMPQNPWESCMDAEPEH

分子量

26.6 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

CACNG1 Protein, Mouse (Cell-Free, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CACNG1 Protein, Mouse (Cell-Free, His)
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HY-P702229
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