1. 重组蛋白
  2. CD Antigens Complement System
  3. Platelet CD Proteins Erythrocyte CD Proteins Epithelial cell CD Proteins Endothelial cell CD Proteins Complement Regulatory Proteins
  4. DAF Protein/CD55 Decay Accelerating Factor (DAF)
  5. CD55/DAF 蛋白, Rat (HEK293, Fc)

CD55/DAF 蛋白, Rat (HEK293, Fc)

目录号: HY-P76241
COA 产品使用指南

CD55/DAF 蛋白缺乏对特征注释传播至关重要的保守残基,并表现出独特的品质,促使人们探索其结构和功能含义。这种独特的性质表明分子相互作用和调节机制中存在潜在的细微差别。CD55/DAF 蛋白, Rat (HEK293, Fc) 是重组的 CD55/DAF 蛋白,由 HEK293 表达,带有 C-hFc 标签。CD55/DAF 蛋白, Rat (HEK293, Fc) 全长 371 个氨基酸,分子量约为 75-95 KDa。

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10 μg ¥1000 In-stock
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100 μg ¥3800 In-stock
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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The CD55/DAF protein lacks conserved residues critical for feature annotation propagation and exhibits unique qualities that have prompted exploration of its structural and functional implications. This unique property suggests underlying nuances in molecular interactions and regulatory mechanisms. CD55/DAF Protein, Rat (HEK293, Fc) is the recombinant rat-derived CD55/DAF protein, expressed by HEK293 , with C-hFc labeled tag. The total length of CD55/DAF Protein, Rat (HEK293, Fc) is 371 a.a., with molecular weight of 75-95 KDa.

研究背景

CD55,也称为衰变加速因子(DAF),表现出独特的特征,因为它缺乏特征注释传播所必需的保守残基。这种独特的品质促使人们进一步探索 CD55 的结构和功能含义,强调其分子相互作用和调控机制中潜在的细微差别。这些保守残基的缺失强调了 CD55 的特殊性,并为揭示其在免疫调节和补体系统调节中的特定作用提供了有趣的研究途径。

生物活性

Immobilized Rat CD55 at 2 μg/mL (100 μL/well) can bind CD55 antibody. The ED50 for this effect is 1.221 μg/mL.

种属

Rat

表达系统

HEK293

标签

C-hFc

蛋白编号

Q9Z0M0 (M1-S371)

基因 ID

64036  [NCBI]

蛋白结构
N-term
DAF (M1-S371)
Accession # Q9Z0M0
hFc
C-term
同用名
Complement Decay-Accelerating factor; CD55; CR; DAF
氨基酸序列

DCGPPPDIPNATPNSGKHTKFAHQSQVTYSCNKGFKQIPDKPNTVVCLENDQWSNYETFCNKTCSAPLRLNYASLKKEYRNMNFFPIGTTVEFECRPGFQKVTSRTGKSTCLEELVWSPVVEFCKKRACPNPRELKNGHINIPTDILFGSEIIFSCDKGYKLVGVTSTFCSITGNAVDWDDVFPVCTTIFCPDPPKINNGRIREESDSYAYRQSVTYSCDQGFILVGNSSVHCTLNGEVGEWSSAPPKCIERHKVPTKTPPEVDIPSTMPHESTLINFPSTRAPLSHKPTTVKVPGTRVKPTSHKPTEVKVPATQHVPVSKTTVRHPTRTSKDRGES

分子量

Approximately 75-95 kDa due to the glycosylation.

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CD55/DAF Protein, Rat (HEK293, Fc)
目录号:
HY-P76241
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