DNA helicase II/uvrD 蛋白, E.coli

DNA helicase II/uvrD protein, a DNA-dependent ATPase, efficiently unwinds DNA duplexes with 3' to 5' polarity, especially from nicked substrates. It actively participates in post-incision stages of nucleotide excision repair and methyl-directed mismatch repair, contributing to genomic integrity by facilitating DNA unwinding in various repair mechanisms. DNA helicase II/uvrD Protein, E.coli is the recombinant E. coli-derived DNA helicase II/uvrD protein, expressed by E. coli, with tag free. The total length of DNA helicase II/uvrD Protein, E.coli is 720 a.a., with molecular weight of ~82.0 kDa.

DNA 解旋酶 II/uvrD 蛋白是一种具有 DNA 依赖性 ATP 酶活性的解旋酶。它通过解开与结合链有关的 3' 至 5' 极性的 DNA 双链体来发挥作用，与双链双链体 DNA 相比，它在从带切口的底物启动解链方面表现出更高的效率。该蛋白质积极参与核苷酸切除修复和甲基定向错配修复的切口后阶段。此外，它可能参与烷基化 DNA 的修复，表明它在各种 DNA 修复机制中具有多种作用。DNA 解旋酶 II/uvrD 的协调活动通过促进 DNA 结构的解旋并参与与 DNA 修复相关的重要细胞过程，有助于维持基因组完整性。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

E.coli

E. coli

Tag Free

P03018 (M1-V720)

66672280  [NCBI]

MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIAWLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHHMDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQSYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNILVDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAETIRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVVNRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDALSYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKALAGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIENLEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLEFPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKEVYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFGEGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV

Approximately 82.0 kDa

• 
  Greater than 91% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from 0.22 μm filtered solution in PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.