E5 蛋白, Human papillomavirus type 16 (Cell-Free, His, GST)

E5 protein maintains host cells in a proliferation-competent state by enhancing host epidermal growth factor receptor (EGFR) activation and inhibiting EGFR internalization. It redistributes host caveolin-1 and glycosphingolipid (ganglioside GM1) to the plasma membrane, potentially facilitating immune evasion and cell proliferation. E5 also interacts with vacuolar H+-ATPase, modulating endosomal pH, and disrupts major histocompatibility class I transport, retaining it in the Golgi. Existing as a homooligomer, E5 interacts with host BCAP31, ATP6V0C, and HLA class I, inhibiting the host immune response. E5 Protein, Human papillomavirus type 16 (Cell-Free, His, GST) is the recombinant Virus-derived E5 protein, expressed by E. coli Cell-free, with N-10*His, C-GST labeled tag. The total length of E5 Protein, Human papillomavirus type 16 (Cell-Free, His, GST) is 83 a.a., with molecular weight of 36.4 kDa.

E5 蛋白通过增强宿主表皮生长因子受体 (EGFR) 激活并在 EGF 刺激后抑制 EGFR 内化，维持宿主细胞在分化时处于有增殖能力的状态。它诱导脂质筏的宿主 Caveolin-1 和鞘糖脂（神经节苷脂 GM1）成分重新分布到质膜。鉴于 GM1 抑制细胞毒性 T 淋巴细胞、阻止免疫突触形成并增强 EGFR 的增殖信号传导，E5 可能通过共享机制促进免疫逃避和细胞增殖。E5 还通过与液泡 H+-ATP 酶（一种负责酸化细胞器的质子泵）相互作用来调节内体 pH 值。此外，E5 会破坏主要组织相容性 I 类向细胞表面的运输，从而将复合物保留在高尔基体中。E5 作为同源寡聚物存在，与宿主 BCAP31 相互作用，与 HPV 阳性分化细胞维持增殖的能力相关。此外，E5 与宿主 ATP6V0C 和 HLA I 类重链 A1、A2、A3 和 B8 结合，通过将 MHC I 类肽隔离到宿主高尔基体来抑制宿主免疫反应。

Virus

E. coli Cell-free

N-10*His;C-GST

P06927 (M1-T83)

1489077

MTNLDTASTTLLACFLLCFCVLLCVCLLIRPLLLSVSTYTSLIILVLLLWITAASAFRCFIVYIIFVYIPLFLIHTHARFLIT

36.4 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.