1. 重组蛋白
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. EGLP/GPX5 蛋白, Pig (P.pastoris, Myc, His)

EGLP/GPX5 蛋白, Pig (P.pastoris, Myc, His)

目录号: HY-P71739
产品使用指南

EGLP/GPX5可能构成类似于谷胱甘肽过氧化物酶的保护系统,保护精子膜脂质免受过氧化损伤。尽管酶活性有限,但保护作用表明其作用超出了酶的功能。EGLP/GPX5 蛋白, Pig (P.pastoris, Myc, His) 是重组的 EGLP/GPX5 蛋白,由 P. pastoris 表达,带有 N-His, C-Myc 标签。EGLP/GPX5 蛋白, Pig (P.pastoris, Myc, His) 全长 198 个氨基酸,分子量约为 ~26.1 kDa。

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

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描述

EGLP/GPX5 may constitute a protective system similar to glutathione peroxidase, protecting sperm membrane lipids from peroxidative damage. Despite the limited enzyme activity, the protective effect suggests an effect beyond enzyme function. EGLP/GPX5 Protein, Pig (P.pastoris, Myc, His) is the recombinant pig-derived EGLP/GPX5 protein, expressed by P. pastoris , with N-His, C-Myc labeled tag. The total length of EGLP/GPX5 Protein, Pig (P.pastoris, Myc, His) is 198 a.a., with molecular weight of ~26.1 kDa.

研究背景

EGLP/GPX5 蛋白作为类似于谷胱甘肽过氧化物酶的保护系统的潜在组成部分出现,保护精子膜脂质免受过氧化物损伤。尽管纯化的猪酶对过氧化氢或有机氢过氧化物表现出有限的酶活性,但其保护作用表明其作用超出了酶的功能。相反,EGLP/GPX5 可以通过与脂质过氧化物结合来保护精子免受附睾中过早顶体反应的影响。这种结合作用可以阻止脂质过氧化物与磷脂酶 A2 的相互作用,从而减轻顶体反应的诱导。EGLP/GPX5 的多方面性质意味着它参与非酶机制,有助于防御过氧化物诱导的损伤,突显其在保护精子活力和功能方面的潜在意义。进一步的研究对于揭示 EGLP/GPX5 在这种保护能力中所协调的特定分子途径和相互作用至关重要。

种属

Pig

表达系统

P. pastoris

标签

N-His;C-Myc

蛋白编号

O18994 (22N-219E)

基因 ID

396920  [NCBI]

蛋白结构
N-term
6*His
EGLP (22N-219E)
Accession # O18994
Myc
C-term
同用名
GPX5; Epididymal secretory glutathione peroxidase; EGLP; Glutathione peroxidase 5; GPx-5; GSHPx-5
氨基酸序列

NSNLEKMDCYKDVTGTIYDYDAFTLNGNEHIQFKQYAGKHVLFVNVATYCGLTAQYPELNTLQEELKPFGLVVLGFPCNQFGKQEPGENSEILLGLKYVRPGGGYVPNFQLFEKGDVNGEKEQKVFTFLKHSCPHPSELIGSIGYISWEPIRVHDIRWNFEKFLVGPDGVPVMRWVHETPISTVKSDILAYLKQFKTE

分子量

Approximately 26.1 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

EGLP/GPX5 Protein, Pig (P.pastoris, Myc, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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EGLP/GPX5 Protein, Pig (P.pastoris, Myc, His)
目录号:
HY-P71739
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