Ephrin B3 蛋白, Mouse (HEK293, His)

Ephrin B3 protein, a transmembrane ligand, binds adjacent Eph receptors, triggering bidirectional signaling. It induces axon collapse in vitro and may influence axon guidance and orientation. Ephrin B3 interacts with GRIP1 and GRIP2, suggesting regulatory roles in development and cellular functions. Ephrin B3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Ephrin B3 protein, expressed by HEK293 , with C-His labeled tag. The total length of Ephrin B3 Protein, Mouse (HEK293, His) is 200 a.a., with molecular weight of ~23.5 kDa.

Ephrin B3 蛋白是 Eph 受体的细胞表面跨膜配体，对神经元、血管和上皮发育至关重要，通过与相邻细胞上的 Eph 受体混杂结合来参与接触依赖性双向信号传导。这导致受体下游的正向信号传导和肝配蛋白配体下游的反向信号传导。Ephrin B3 对前脑功能具有潜在意义，在体外与连合轴突/生长锥结合并诱导其塌陷，表明其在轴突引导中发挥作用。此外，它可能有助于限制纵向突出轴突的方向。该蛋白与 GRIP1 和 GRIP2 相互作用，进一步强调其参与复杂的信号传导过程以及发育和细胞功能过程中的潜在调节作用。

Immobilized mouse EFNB3-His at 10μg/mL (100μL/well) can bind biotinylated mouse EPHB3, the ED50 for this effect is 0.5188 μg/mL.

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  Immobilized mouse EFNB3-His at 10μg/mL (100μL/well) can bind biotinylated mouse EPHB3, the ED₅₀ for this effect is 0.5188 μg/mL.

Mouse

HEK293

C-His

O35393 (L28-A227)

13643  [NCBI]

LSLEPVYWNSANKRFQAEGGYVLYPQIGDRLDLLCPRARPPGPHSSPSYEFYKLYLVEGAQGRRCEAPPAPNLLLTCDRPDLDLRFTIKFQEYSPNLWGHEFRSHHDYYIIATSDGTREGLESLQGGVCLTRGMKVLLRVGQSPRGGAVPRKPVSEMPMERDRGAAHSAEPGRDTIPGDPSSNATSRGAEGPLPPPSMPA

Approximately 23.5 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.