Erythropoietin/EPO 蛋白, Mouse (HEK293)

This gene encodes the erythropoietin (EPO) protein that is critical for regulating erythropoiesis. It is mainly expressed in the liver during fetal development and is transferred to the kidneys in adults. Erythropoietin/EPO Protein, Mouse (HEK293) is the recombinant mouse-derived Erythropoietin/EPO protein, expressed by HEK293 , with tag free. The total length of Erythropoietin/EPO Protein, Mouse (HEK293) is 166 a.a., with molecular weight of ~23-36 kDa.

该基因编码糖蛋白激素促红细胞生成素，在调节红细胞生成和血红蛋白生物合成中发挥着至关重要的作用。虽然该基因在胎儿发育期间主要在肝脏中表达，但在成人中则转移到肾脏。编码蛋白的完全缺失会导致小鼠胚胎致死性贫血，而成年小鼠的条件性失活会导致慢性、正细胞性和正色素性贫血。表达该基因的人类直系同源物的转基因小鼠表现出红细胞增多症。通过选择性剪接产生各种同种型。值得注意的是，该基因在参考数据集中的表达量较低。

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED₅₀ for this effect is 0.2134 ng/mL, corresponding to a specific activity is 4.68×10^6 units/mg.

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  Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells.The ED50 for this effect is 0.2134 ng/ml.corresponding to a specific activity is 4.68×10⁶units/mg.

Mouse

HEK293

Tag Free

NP_031968.1 (A27-R192)

13856

APPRLICDSRVLERYILEAKEAENVTMGCAEGPRLSENITVPDTKVNFYAWKRMEVEEQAIEVWQGLSLLSEAILQAQALLANSSQPPETLQLHIDKAISGLRSLTSLLRVLGAQKELMSPPDTTPPAPLRTLTVDTFCKLFRVYANFLRGKLKLYTGEVCRRGDR

Approximately 23-36 kDa due to glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.