FGF-1 蛋白, Canine

The FGF-1 protein is critical in cellular regulation, coordinating key processes such as cell survival, division, angiogenesis, differentiation and migration. It exhibits potent mitogenic properties in vitro and serves as a ligand for FGFR1 and integrins. FGF-1 Protein, Canine is the recombinant canine-derived FGF-1 protein, expressed by E. coli , with tag free. The total length of FGF-1 Protein, Canine is 140 a.a., with molecular weight of ~16 kDa.

FGF-1 蛋白在调节细胞存活、分裂、血管生成、分化和迁移等重要细胞过程中发挥着关键作用。在体外，它表现出有效的有丝分裂特性，并可作为 FGFR1 和整合素的配体。当存在肝素时，FGF-1 与 FGFR1 结合，通过酪氨酸残基的自磷酸化导致 FGFR1 的二聚化和激活。这些磷酸化残基充当相互作用蛋白质的对接位点，启动各种信号级联反应。FGF-1 还与整合素结合，并与整合素和 FGFR1 形成三元复合物，这对于 FGF-1 信号转导至关重要。

Measured in a cell proliferation assay using NIH-3T3 mouse fibroblast cells. The ED₅₀ for this effect is 0.2335 ng/mL, corresponding to a specific activity is 4.28×10⁶ units/mg.

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  Measured in a cell proliferation assay using NIH-3T3 mouse fibroblast cells. The ED₅₀ for this effect is 0.2335 ng/mL, corresponding to a specific activity is 4.28×10⁶ units/mg.

Canine

E. coli

Tag Free

J9NTP4 (F16-D155)

607724  [NCBI]

FNLPPGNYMKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHIQLQLSAESVGEVYIKSTETGQYLAMDTDGLLYGSQTPNEECLFLERLEENHYNTYTSKKHAEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPLPVSSD

Approximately 16 kDa

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  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.