Fibrillin-1/Asprosin 蛋白, Mouse (HEK293, His)

Fibrillin-1/Asprosin proteins play crucial roles in a variety of biological processes. They regulate osteoblast maturation by controlling the availability of TGF-β and regulating TGF-β and BMP levels. Fibrillin-1/Asprosin Protein, Mouse (HEK293, His) is the recombinant mouse-derived Fibrillin-1/Asprosin protein, expressed by HEK293 , with N-His labeled tag. The total length of Fibrillin-1/Asprosin Protein, Mouse (HEK293, His) is 138 a.a., with molecular weight of ~30.0 kDa.

聚糖成分参与各种生物过程。它们通过控制 TGF-β 的可用性并校准 TGF-β 和 BMP 水平来调节成骨细胞成熟。它们还通过结合和隔离 TNFSF11 来负向调节破骨细胞生成，TNFSF11 是破骨细胞分化和功能的重要因子。这会破坏 TNFSF11 诱导的信号传导并损害转录因子 NFATC1 的激活，而转录因子 NFATC1 对破骨细胞分化很重要。

Measured in a cell proliferation assay using human umbilical vein endothelial cells. The ED₅₀ this effect is 20.33 ng/mL, corresponding to a specific activity is 4.919×10⁴ units/mg.

Mouse

HEK293

N-His

Q61554 (S2734-H2873)

14118  [NCBI]

STNETDASDIQDGSEMEANVSLASWDVEKPASFAFNISHVNNKVRILELLPALTTLMNHNRYLIESGNEDGFFKINQKEGVSYLHFTKKKPVAGTYSLQISSTPLYKKKELNQLEDRYDKDYLSGELGDNLKMKIQILLH

Approximately 30.0 kDa

Greater than 85% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.