FLAD1 蛋白, Human (His-SUMO)

The FLAD1 protein plays a key role in cellular processes involving small nuclear RNA (snRNA) and small nucleolar RNA (snoRNA). It catalyzes two consecutive methylation steps that are necessary to convert the 7-monomethylguanosine (m(7)G) cap of these RNAs into 2,2,7-trimethylguanosine (m(2, 2,7)G) Cap structure is critical. FLAD1 Protein, Human (His-SUMO) is the recombinant human-derived FLAD1 protein, expressed by E. coli , with N-His, N-SUMO labeled tag. The total length of FLAD1 Protein, Human (His-SUMO) is 490 a.a., with molecular weight of ~70.2 kDa.

TGS1 蛋白在涉及小核 RNA (snRNA) 和小核仁 RNA (snoRNA) 的细胞过程中充当关键催化剂。它协调两个连续的甲基化步骤，这对于将这些 RNA 的 7-单甲基鸟苷 (m(7)G) 帽转化为 2,2,7-三甲基鸟苷 (m(2,2,7)G) 帽结构至关重要。该酶对鸟嘌呤表现出特异性，N7 甲基化是 N2 甲基化的先决条件。值得注意的是，U snRNA m7G 帽的高甲基化诱导其定位于核灶、与线圈共定位以及典型卡哈尔小体 (CB) 的形成，突出了其在细胞调节中的作用。

Human

E. coli

N-His;N-SUMO

Q8NFF5 (1M-490T)

80308  [NCBI]

MTSRASELSPGRSVTAGIIIVGDEILKGHTQDTNTFFLCRTLRSLGVQVCRVSVVPDEVATIAAEVTSFSNRFTHVLTAGGIGPTHDDVTFEAVAQAFGDELKPHPKLEAATKALGGEGWEKLSLVPSSARLHYGTDPCTGQPFRFPLVSVRNVYLFPGIPELLRRVLEGMKGLFQNPAVQFHSKELYVAADEASIAPILAEAQAHFGRRLGLGSYPDWGSNYYQVKLTLDSEEEGPLEECLAYLTARLPQGSLVPYMPNAVEQASEAVYKLAESGSSLGKKVAGALQTIETSLAQYSLTQLCVGFNGGKDCTALLHLFHAAVQRKLPDVPNPLQILYIRSISPFPELEQFLQDTIKRYNLQMLEAEGSMKQALGELQARHPQLEAVLMGTRRTDPYSCSLCPFSPTDPGWPAFMRINPLLDWTYRDIWDFLRQLFVPYCILYDRGYTSLGSRENTVRNPALKCLSPGGHPTYRPAYLLENEEEERNSRT

Approximately 70.2 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.