2. 重组蛋白
  3. Enzymes & Regulators
  4. Oxidoreductases (EC 1)
  5. gdh2 蛋白, Saccharolobus solfataricus (His, Strep)

gdh2 蛋白, Saccharolobus solfataricus (His, Strep)

目录号: HY-P701896
产品使用指南

gdh2 蛋白在 D-葡萄糖通过葡萄糖酸内酯依赖 NAD(P)(+) 氧化为 D-葡萄糖酸过程中充当催化剂。它具有多功能性，可以利用 NAD(+) 和 NADP(+) 作为电子受体。gdh2 在葡萄糖降解中发挥着关键作用，特别是通过 Entner-Doudoroff 途径的非磷酸化变体。gdh2 蛋白, Saccharolobus solfataricus (His, Strep) 是重组的 gdh2 蛋白，由 E. coli 表达，带有 N-Strep, N-6*His 标签。gdh2 蛋白, Saccharolobus solfataricus (His, Strep) 全长 367 个氨基酸。

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描述

gdh2 protein acts as a catalyst in the NAD(P)(+)-dependent oxidation of D-glucose to D-gluconate through gluconolactone. Demonstrating versatility, it can utilize both NAD(+) and NADP(+) as electron acceptors. gdh2 plays a pivotal role in the degradation of glucose, specifically through a non-phosphorylative variant of the Entner-Doudoroff pathway. gdh2 Protein, Saccharolobus solfataricus (His, Strep) is the recombinant gdh2 protein, expressed by E. coli , with N-Strep, N-6*His labeled tag. The total length of gdh2 Protein, Saccharolobus solfataricus (His, Strep) is 367 a.a., .
研究背景

gdh2 蛋白作为一种酶，利用葡萄糖酸内酯作为中间体，催化 D-葡萄糖向 D-葡萄糖酸的 NAD(P)(+) 依赖性氧化。值得注意的是，这种酶用途广泛，可以在反应中使用 NAD(+) 和 NADP(+) 作为电子受体。它参与 Entner-Doudoroff 途径的非磷酸化变体，凸显了它在葡萄糖降解中的作用，有助于细胞能量代谢和碳利用。
种属

Others
表达系统

E. coli
标签

N-Strep;N-6*His
蛋白编号

Q97UH6 (K2-E368)
基因 ID

72911600
蛋白结构
N-term
Strep-6*His
gdh2 (K2-E368)
Accession # Q97UH6
C-term
同用名
gdh2; Glucose 1-dehydrogenase 2; GDH 2; GlcDH 2
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

gdh2 Protein, Saccharolobus solfataricus (His, Strep) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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