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  4. GJD2 蛋白, Bovine (Cell-Free, His, SUMO)

GJD2 蛋白, Bovine (Cell-Free, His, SUMO)

目录号: HY-P702287
产品使用指南

GJD2 蛋白是一种连接蛋白，有助于间隙连接的形成，其中连接子创建跨膜通道。这些通道通过促进低分子量材料在相邻细胞之间的扩散来实现直接的细胞间通讯。每个六聚体连接子由连接蛋白组成，在间隙连接簇内相邻细胞之间交换必需物质方面发挥着至关重要的作用。GJD2 蛋白, Bovine (Cell-Free, His, SUMO) 是重组的 GJD2 蛋白，由 E. coli Cell-free 表达，带有 N-6*His, N-SUMO 标签。GJD2 蛋白, Bovine (Cell-Free, His, SUMO) 全长 321 个氨基酸，分子量为 49.1 kDa。

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描述

GJD2 Protein, a connexin, aids in gap junction formation, where connexons create transmembrane channels. These channels allow direct intercellular communication by facilitating the diffusion of low molecular weight materials between adjacent cells. Each hexameric connexon, composed of connexins, plays a crucial role in exchanging essential substances among neighboring cells within gap junction clusters. GJD2 Protein, Bovine (Cell-Free, His, SUMO) is the recombinant bovine-derived GJD2 protein, expressed by E. coli Cell-free , with N-6*His, N-SUMO labeled tag. The total length of GJD2 Protein, Bovine (Cell-Free, His, SUMO) is 321 a.a., with molecular weight of 49.1 kDa.
研究背景

GJD2 蛋白作为连接蛋白，有助于间隙连接的形成，间隙连接由紧密堆积的跨膜通道对（称为连接子）组成。这些连接子促进低分子量材料从一个细胞扩散到相邻细胞，从而实现直接的细胞间通讯。每个连接子都是由连接蛋白六聚体构成的，这些复杂的结构在间隙连接簇内相邻细胞之间的必需物质交换中发挥着至关重要的作用。
种属

Bovine
表达系统

E. coli Cell-free
标签

N-6*His;N-SUMO
蛋白编号

Q866T7 (M1-V321)
基因 ID

282577
蛋白结构
N-term
6*His-SUMO
GJD2 (M1-V321)
Accession # Q866T7
C-term
同用名
Gap junction delta-2 protein; Connexin-36; Cx36; Gap junction alpha-9 protein
氨基酸序列

MGEWTILERLLEAAVQQHSTMIGRILLTVVVIFRILIVAIVGETVYDDEQTMFVCNTLQPGCNQACYDRAFPISHIRYWVFQIIMVCTPSLCFITYSVHQSAKQRERRYSTVFLALDRDPPESMGGPGGTGGGGSGGGKREDKKLQNAIVNGVLQNTENTSKETEPDCLEVKELTPHPSGLRTASRSKLRRQEGISRFYIIQVVFRNALEIGFLVGQYFLYGFSVPGLYECDRYPCIKEVECYVSRPTEKTVFLVFMFAVSGICVVLNLAELNHLGWRKIKLAVRGAQAKRKSVYEIRNKDLPRVSVPNFGRTQSSDSAYV

分子量

49.1 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

GJD2 Protein, Bovine (Cell-Free, His, SUMO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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