1. 重组蛋白
  2. Viral Proteins
  3. GP2b 蛋白, PRRSV (Cell-Free, His)

GP2b 蛋白, PRRSV (Cell-Free, His)

目录号: HY-P702299
产品使用指南

GP2b 蛋白是一种次要包膜蛋白,可能充当病毒孔蛋白,帮助病毒脱壳,将基因组 RNA 释放到细胞质中。它形成同源低聚物,产生二聚体、三聚体和四聚体等高阶结构。GP2b 可能是 GP2a-GP3-GP4 复合体的一部分,有助于病毒包膜的整体功能。GP2b 蛋白, PRRSV (Cell-Free, His) 是重组的 GP2b 蛋白,由 E. coli Cell-free 表达,带有 N-10*His 标签。GP2b 蛋白, PRRSV (Cell-Free, His) 全长 69 个氨基酸,分子量为 13.7 kDa。

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描述

GP2b Protein, a minor envelope protein, potentially acts as a viroporin, aiding virus uncoating for genomic RNA release into the cytoplasm. It forms homooligomers, creating higher-order structures like dimers, trimers, and tetramers. GP2b is likely part of the GP2a-GP3-GP4 complex, contributing to the overall functionality of the viral envelope. GP2b Protein, PRRSV (Cell-Free, His) is the recombinant Virus-derived GP2b protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of GP2b Protein, PRRSV (Cell-Free, His) is 69 a.a., with molecular weight of 13.7 kDa.

研究背景

GP2b 蛋白是一种次要包膜蛋白,可能充当病毒颗粒包膜内的病毒孔蛋白,促进病毒脱壳,将基因组 RNA 释放到细胞质中,这是后续复制的关键步骤。它形成同源低聚物并与其自身缔合以形成高阶结构,包括二聚体、三聚体和四聚体。此外,GP2b 可能是 GP2a-GP3-GP4 复合物的一部分,有助于病毒包膜的整体功能。

种属

Virus

表达系统

E. coli Cell-free

标签

N-10*His

蛋白编号

P0C6Y6 (G2-L70)

基因 ID

/

蛋白结构
N-term
10*His
GP2b (G2-L70)
Accession # P0C6Y6
C-term
同用名
Envelope small membrane protein; Glycoprotein 2b; Protein GP2b; Gs
氨基酸序列

GSLWSKISQLFVDAFTEFLVSVVDIAIFLAILFGFTVAGWLLVFLLRVVCSALLRSRSAIHSPELSKVL

分子量

13.7 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

GP2b Protein, PRRSV (Cell-Free, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
GP2b Protein, PRRSV (Cell-Free, His)
目录号:
HY-P702299
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