2. 重组蛋白
  3. Receptor Proteins
  4. G-Protein-Coupled Receptors (GPCRs)
  5. GPR87 蛋白, Human (Cell-Free, Strep)

GPR87 蛋白, Human (Cell-Free, Strep)

目录号: HY-P702309
产品使用指南

GPR87 是一种溶血磷脂酸 (LPA) 受体，可响应 DNA 损伤而至关重要地激活 p53/TP53 依赖性生存途径。它与 LPA 的相互作用在介导细胞对 DNA 损伤的反应中发挥着关键作用，突出了它在细胞存活和 DNA 损伤修复过程中的重要性。GPR87 蛋白, Human (Cell-Free, Strep) 是重组的 GPR87 蛋白，由 E. coli Cell-free 表达，带有 C-Twin-Strep 标签。GPR87 蛋白, Human (Cell-Free, Strep) 全长 358 个氨基酸，分子量为 44.5 kDa。

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描述

GPR87, a lysophosphatidic acid (LPA) receptor, crucially activates p53/TP53-dependent survival pathways in response to DNA damage. Its interaction with LPA plays a key role in mediating cellular responses to DNA damage, highlighting its significance in cell survival and DNA damage repair processes. GPR87 Protein, Human (Cell-Free, Strep) is the recombinant human-derived GPR87 protein, expressed by E. coli Cell-free , with C-Twin-Strep labeled tag. The total length of GPR87 Protein, Human (Cell-Free, Strep) is 358 a.a., with molecular weight of 44.5 kDa.
研究背景

GPR87 是溶血磷脂酸 (LPA) 的受体，在响应 DNA 损伤而激活的 p53/TP53 依赖性生存途径中发挥着至关重要的作用。该受体与 LPA 的结合有助于介导细胞对 DNA 损伤的反应，强调了其在与细胞存活和 DNA 损伤修复相关的过程中的重要性。
种属

Human
表达系统

E. coli Cell-free
标签

C-Twin-Strep
蛋白编号

Q9BY21 (M1-V358)
基因 ID

53836
蛋白结构
N-term
GPR87 (M1-V358)
Accession # Q9BY21
Twin-Strep
C-term
同用名
G-protein coupled receptor 87; G-protein coupled receptor 95
氨基酸序列

MGFNLTLAKLPNNELHGQESHNSGNRSDGPGKNTTLHNEFDTIVLPVLYLIIFVASILLNGLAVWIFFHIRNKTSFIFYLKNIVVADLIMTLTFPFRIVHDAGFGPWYFKFILCRYTSVLFYANMYTSIVFLGLISIDRYLKVVKPFGDSRMYSITFTKVLSVCVWVIMAVLSLPNIILTNGQPTEDNIHDCSKLKSPLGVKWHTAVTYVNSCLFVAVLVILIGCYIAISRYIHKSSRQFISQSSRKRKHNQSIRVVVAVFFTCFLPYHLCRIPFTFSHLDRLLDESAQKILYYCKEITLFLSACNVCLDPIIYFFMCRSFSRRLFKKSNIRTRSESIRSLQSVRRSEVRIYYDYTDV

分子量

44.5 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

GPR87 Protein, Human (Cell-Free, Strep) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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