Granzyme B/GZMB 蛋白, Human (HEK293, His)

Granzyme B (GZMB) is a cytosolic protease abundant in cytotoxic T-cells and natural killer cells, pivotal for immune defense. Delivered into target cells, GZMB activates caspase-independent pyroptosis by cleaving gasdermin-E (GSDME). This triggers pyroptosis, leading to target cell death. GZMB exhibits substrate specificity for aspartate residue cleavage and contributes to an activation cascade of caspases (3, 9, 10), impacting apoptosis execution. Involved in bacterial infection response, GZMB cleaves and activates caspase-7, promoting plasma membrane repair. Its multifaceted activities underscore its crucial role in immune responses and cellular defense. Granzyme B/GZMB Protein, Human (HEK293, His) is the recombinant human-derived Granzyme B/GZMB protein, expressed by HEK293, with C-His labeled tag. The total length of Granzyme B/GZMB Protein, Human (HEK293, His) is 247 a.a., with molecular weight of 35-40 kDa.

粒酶 B (GZMB) 是一种高度丰富的蛋白酶，存在于细胞毒性 T 细胞和自然杀伤细胞的胞质颗粒中，在免疫防御机制中发挥着关键作用。通过免疫突触递送至靶细胞后，GZMB 通过催化gasdermin-E (GSDME) 的裂解来激活不依赖半胱天冬酶的细胞焦亡。这导致 GSDME 的成孔部分释放，引发细胞焦亡并最终导致靶细胞死亡。GZMB 对天冬氨酸残基后的裂解表现出底物特异性。此外，它还参与 caspase 的激活级联，包括 caspase-3、-9 和 -10，从而有助于细胞凋亡的执行。此外，GZMB 参与对细菌感染的反应，它裂解并激活 caspase-7，促进质膜修复。GZMB 的多方面活动凸显了其在协调免疫反应和细胞防御机制中的关键作用。

Measured by its ability to cleave a peptide substrate, t-Butyloxycaronyl-Ala-Ala-Asp-ThioBenzylester (Boc-AAD-SBzl), in the presence of 5,5’-Dithio-bis (2-nitrobenzoic acid) (DTNB) and the specific activity is ≥1500 pmol/min/μg. Note: The enzyme needs to be activated by Active Cathepsin C.

Human

HEK293

C-His

P10144 (M1-Y247)

3002  [NCBI]

35-40 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.