GRIN1 蛋白, Human (His)

The GRIN1 protein is a component of the NMDA receptor complex that forms heterotetrameric ligand-gated ion channels with high calcium permeability. GRIN1 Protein, Human (His) is the recombinant human-derived GRIN1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of GRIN1 Protein, Human (His) is 541 a.a., with molecular weight of ~64.6 kDa.

GRIN1 是 NMDA 受体复合物的关键成分，参与异四聚体配体门控离子通道的构成，该离子通道表现出高钙渗透性和对镁的电压依赖性敏感性。通道激活取决于神经递质结合的复杂相互作用，谷氨酸与ε亚基结合，甘氨酸与zeta亚基结合，结合膜去极化以减轻Mg(2+)对通道的抑制。对谷氨酸的敏感性以及通道的动力学取决于特定的亚基组成。GRIN1 形成异四聚体通道，通常由两个 zeta 亚基和两个 epsilon 亚基组成，其中后者可以是 GRIN2A、GRIN2B、GRIN2C 或 GRIN2D。此外，它还可以参与 GRIN3A 或 GRIN3B 通道的形成。体内动态亚基组成进一步强调了 GRIN1 参与 NMDA 受体功能的复杂性调节。此外，GRIN1 参与各种蛋白质相互作用，例如与 SNX27、DLG4、MPDZ、LRFN1、LRFN2、MYZAP 和 PRR7 的相互作用，有助于其在细胞过程中发挥多方面的作用。

Human

E. coli

N-6*His

Q05586 (R19-Q559)

2902  [NCBI]

RAACDPKIVNIGAVLSTRKHEQMFREAVNQANKRHGSWKIQLNATSVTHKPNAIQMALSVCEDLISSQVYAILVSHPPTPNDHFTPTPVSYTAGFYRIPVLGLTTRMSIYSDKSIHLSFLRTVPPYSHQSSVWFEMMRVYSWNHIILLVSDDHEGRAAQKRLETLLEERESKAEKVLQFDPGTKNVTALLMEAKELEARVIILSASEDDAATVYRAAAMLNMTGSGYVWLVGEREISGNALRYAPDGILGLQLINGKNESAHISDAVGVVAQAVHELLEKENITDPPRGCVGNTNIWKTGPLFKRVLMSSKYADGVTGRVEFNEDGDRKFANYSIMNLQNRKLVQVGIYNGTHVIPNDRKIIWPGGETEKPRGYQMSTRLKIVTIHQEPFVYVKPTLSDGTCKEEFTVNGDPVKKVICTGPNDTSPGSPRHTVPQCCYGFCIDLLIKLARTMNFTYEVHLVADGKFGTQERVNNSNKKEWNGMMGELLSGQADMIVAPLTINNERAQYIEFSKPFKYQGLTILVKKEIPRSTLDSFMQPFQ

Approximately 64.6 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.